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Characterization of an 'Amyloid Only' Transgenic (B6C3-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax) Mouse Model of Alzheimer's Disease.
Finnie, G S; Gunnarsson, R; Manavis, J; Blumbergs, P C; Mander, K A; Edwards, S; Van den Heuvel, C; Finnie, J W.
Afiliación
  • Finnie GS; Cairns Clinical School, College of Medicine and Dentistry, James Cook University, Townsville, Queensland, Australia; NH&MRC Australian Centre for Electromagnetic Bioeffects Research, Adelaide, South Australia, Australia.
  • Gunnarsson R; Cairns Clinical School, College of Medicine and Dentistry, James Cook University, Townsville, Queensland, Australia; Research and Development Centre, Sodra Alvsborg Narhalsan, Primary Health Care, Vastra Gotaland, Sweden; Department of Public Health and Community Medicine, The Sahlgrenska Academy, U
  • Manavis J; University of Adelaide School of Medicine, Adelaide, South Australia, Australia.
  • Blumbergs PC; SA Pathology Hanson Institute, Centre for Neurological Diseases, Adelaide, South Australia, Australia; University of Adelaide School of Medicine, Adelaide, South Australia, Australia; NH&MRC Australian Centre for Electromagnetic Bioeffects Research, Adelaide, South Australia, Australia.
  • Mander KA; University of Adelaide School of Medicine, Adelaide, South Australia, Australia; NH&MRC Australian Centre for Electromagnetic Bioeffects Research, Adelaide, South Australia, Australia.
  • Edwards S; Data, Design and Statistics Service, School of Public Health, University of Adelaide, Adelaide, South Australia, Australia.
  • Van den Heuvel C; University of Adelaide School of Medicine, Adelaide, South Australia, Australia.
  • Finnie JW; SA Pathology Hanson Institute, Centre for Neurological Diseases, Adelaide, South Australia, Australia; University of Adelaide School of Medicine, Adelaide, South Australia, Australia; NH&MRC Australian Centre for Electromagnetic Bioeffects Research, Adelaide, South Australia, Australia. Electron
J Comp Pathol ; 156(4): 389-399, 2017 May.
Article en En | MEDLINE | ID: mdl-28431735
The spatiotemporal pattern of cerebral amyloid deposition, detectable as light microscopically recognizable aggregates in an 'amyloid only' transgenic mouse model of Alzheimer's disease, B6C3-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax, is reported for the first time in this strain. Monoclonal and polyclonal antibodies were used to detect amyloid deposition immunohistochemically in brains collected from these mice at 3-12 months of age. Amyloid aggregates (20-200 µm) were first found in serial, whole coronal sections of brain at 4 months of age and these increased progressively, plateauing at 11-12 months. They were most abundant in the cerebral cortices, hippocampus, olfactory bulbs, some white matter tracts and the cerebellar molecular layer; no amyloid aggregates were found in the midbrain, brainstem or spinal cord, or in an equivalent number of brains from wild-type mice. Since the parahippocampal gyrus is severely damaged early in the clinical course of human Alzheimer's disease, amyloid aggregates were also assessed in this brain region and a similar temporal course of amyloid deposition was observed. Moreover, in this gyrus, the amount of aggregated amyloid showed no significant difference between left- and right-sided gyri. However, the polyclonal antibody detected a significantly greater amyloid burden than the monoclonal antibody at 3-10 months of age and the reverse was seen at 11-12 months of age. The pattern of amyloid deposition in the parahippocampal gyrus also resembled that found in the entire brain over time, when the latter was quantified by the colour deconvolution method, suggesting that this gyrus is a good marker for more widely distributed cerebral amyloid deposition. This neuropathological characterization will permit better use of the B6C3-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax transgenic mouse strain in future studies of Alzheimer's disease pathogenesis, prevention and treatment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Encéfalo / Péptidos beta-Amiloides / Modelos Animales de Enfermedad / Enfermedad de Alzheimer Límite: Animals / Female / Humans Idioma: En Revista: J Comp Pathol Año: 2017 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Encéfalo / Péptidos beta-Amiloides / Modelos Animales de Enfermedad / Enfermedad de Alzheimer Límite: Animals / Female / Humans Idioma: En Revista: J Comp Pathol Año: 2017 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido