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Effect of dalteparin administration on thrombin generation kinetics in healthy dogs.
Gara-Boivin, Carolyn; de Castillo, Jérôme R E; Dunn, Marilyn E; Bédard, Christian.
Afiliación
  • Gara-Boivin C; Department of Pathology and Microbiology, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, Canada.
  • de Castillo JRE; GREPAQ - Department of Veterinary Biomedicine, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, Canada.
  • Dunn ME; Department of Clinical Sciences, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, Canada.
  • Bédard C; Department of Pathology and Microbiology, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, Canada.
Vet Clin Pathol ; 46(2): 269-277, 2017 Jun.
Article en En | MEDLINE | ID: mdl-28430367
BACKGROUND: Dalteparin is used to prevent thrombotic complications in dogs. Measurement of anti-factor Xa (anti-FXa) activity is currently used for monitoring therapy, but remains a nonfunctional test. The calibrated automated thrombogram (CAT) could be a suitable approach for functional monitoring. OBJECTIVES: We hypothesized that the CAT will detect decreased endogenous thrombin potential (ETP) in healthy dogs receiving dalteparin. METHODS: Twenty-four healthy adult Beagles were randomly allocated to 4 equal groups. A single subcutaneous (SC) dose of 50 U/kg, 100 U/kg, or 150 U/kg of dalteparin was given. Platelet-poor plasma (PPP) was collected over a 24-hour period and evaluated by thrombin generation (TG) via CAT, anti-FXa activity, and APTT. Analysis was performed with a repeated-measures general linear mixed model, and the treated groups were compared to a placebo group. RESULTS: Time, dose, and time-dose interaction significantly affected ETP (P < .0001 for all effects), peak (P < .0001 for all effects), rate index (P < .0006 for all effects), and anti-FXa activity (P < .0001 for all effects). No significant time trend was detected in the control group. Dogs receiving the 100 U/kg dalteparin SC injection showed the most homogeneous response of ETP inhibition among treated groups. The % inhibition of ETP from baseline increased nonlinearly as a function of anti-FXa activity (r2 = .8186). CONCLUSIONS: The CAT assay can be employed to measure the effects of dalteparin at different doses in healthy dogs, showing sensitivity to time- and dose-dependent changes in ETP and other TG variables. Further investigation of the CAT as a tool for monitoring low molecular weight heparin therapy in dogs is warranted.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trombina / Dalteparina / Perros / Fibrinolíticos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Vet Clin Pathol Asunto de la revista: MEDICINA VETERINARIA / PATOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trombina / Dalteparina / Perros / Fibrinolíticos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Vet Clin Pathol Asunto de la revista: MEDICINA VETERINARIA / PATOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos