Your browser doesn't support javascript.
loading
Comparative diagnostic efficacy of recombinant LLO and PI-PLC-based ELISAs for detection of listeriosis in animals.
Suryawanshi, Rahul D; Malik, Satya Veer Singh; Jayarao, Bhushan; Chaudhari, Sandeep P; Savage, Emily; Vergis, Jess; Kurkure, Nitin V; Barbuddhe, Sukhadeo B; Rawool, Deepak B.
Afiliación
  • Suryawanshi RD; Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar 243 122, India.
  • Malik SVS; Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar 243 122, India.
  • Jayarao B; Department of Veterinary and Biomedical Sciences, Pennsylvania State University, PA 16801, USA.
  • Chaudhari SP; Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur 440006, India.
  • Savage E; Department of Veterinary and Biomedical Sciences, Pennsylvania State University, PA 16801, USA.
  • Vergis J; Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar 243 122, India.
  • Kurkure NV; Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur 440006, India.
  • Barbuddhe SB; ICAR(-)National Institute of Biotic Stress Management, Raipur 493225, India.
  • Rawool DB; Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar 243 122, India. Electronic address: deepak.rawool@yahoo.com.
J Microbiol Methods ; 137: 40-45, 2017 06.
Article en En | MEDLINE | ID: mdl-28411150
The present study for the first time evaluates the serodiagnostic efficacy of two recombinant antigens namely, listeriolysin O (rLLO) and phosphatidyl-inositol phospholipase C (rPI-PLC). Indirect ELISA with the above recombinant antigens was used on samples collected from bovines (n=106), goats (n=138) and pigs (n=92) having either a history of abortion, emaciation and/or apparently healthy animals. Isolation of Listeria was attempted from the blood samples using USDA-FSIS method. On screening of test sera by rLLO-based ELISA, antibodies against anti-listeriolysin O (ALLO) were observed in goats (22.46%), bovines (15.10%) and pigs (16.31%). As advocated, after adsorption of positive serum samples with streptolysin O (SLO), the seropositivity for ALLO was marginally reduced (p>0.05) in goats (21.73%) and bovines (10.38%), whereas, in pigs the reduction (5.43%) was significant (p<0.05). On the contrary, rPI-PLC-based ELISA revealed higher non-specific seropositivity for antilisterial antibodies in goats (45.65%), bovines (31.13%) and pigs (8.69%). Further, on comparing the seropositivity with isolation rate, of the 16 animals that were culturally-positive for L. monocytogenes, 15 showed ALLO positivity in unadsorbed as well as SLO-adsorbed sera by rLLO-based ELISA, however, rPI-PLC-based ELISA could detect seropositivity in only 5 animals. Moreover, rPI-PLC-based ELISA also showed seropositivity in those animals (7/30) that were culturally positive for other Listeria spp. In conclusion, rLLO can serve as a better antigen than rPI-PLC in ELISA for the serodiagnosis of listeriosis in animals; however, prior adsorption of test sera with SLO is required to avoid false positive results.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxinas Bacterianas / Ensayo de Inmunoadsorción Enzimática / Fosfoinositido Fosfolipasa C / Proteínas de Choque Térmico / Proteínas Hemolisinas / Listeriosis / Enfermedades de los Animales Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: J Microbiol Methods Año: 2017 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxinas Bacterianas / Ensayo de Inmunoadsorción Enzimática / Fosfoinositido Fosfolipasa C / Proteínas de Choque Térmico / Proteínas Hemolisinas / Listeriosis / Enfermedades de los Animales Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: J Microbiol Methods Año: 2017 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos