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The microbiome of the lung and its extracellular vesicles in nonsmokers, healthy smokers and COPD patients.
Kim, Hyun Jung; Kim, You-Sun; Kim, Kang-Hyun; Choi, Jun-Pyo; Kim, Yoon-Keun; Yun, Sunmi; Sharma, Lokesh; Dela Cruz, Charles S; Lee, Jae Seung; Oh, Yeon-Mok; Lee, Sang-Do; Lee, Sei Won.
Afiliación
  • Kim HJ; Department of Internal Medicine, Kyungpook National University School of Medicine, Daegu, Republic of Korea.
  • Kim YS; Department of Pulmonary and Critical Care Medicine, Clinical Research Center for Chronic Obstructive Airway Disease, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
  • Kim KH; Asan Institute for Life Sciences, University of Ulsan College of Medicine, Seoul, Republic of Korea.
  • Choi JP; Asan Institute for Life Sciences, Seoul, Republic of Korea.
  • Kim YK; Asan Institute for Life Sciences, Seoul, Republic of Korea.
  • Yun S; Institute of Convergence Medicine, Ewha Womans University School of Medicine, Seoul, Republic of Korea.
  • Sharma L; Macrogen Inc., Seoul, Republic of Korea.
  • Dela Cruz CS; Section of Pulmonary, Critical Care and Sleep Medicine, Department of Internal Medicine, Yale University, New Haven, CT, USA.
  • Lee JS; Section of Pulmonary, Critical Care and Sleep Medicine, Department of Internal Medicine, Yale University, New Haven, CT, USA.
  • Oh YM; Department of Pulmonary and Critical Care Medicine, Clinical Research Center for Chronic Obstructive Airway Disease, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
  • Lee SD; Department of Pulmonary and Critical Care Medicine, Clinical Research Center for Chronic Obstructive Airway Disease, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
  • Lee SW; Department of Pulmonary and Critical Care Medicine, Clinical Research Center for Chronic Obstructive Airway Disease, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
Exp Mol Med ; 49(4): e316, 2017 04 14.
Article en En | MEDLINE | ID: mdl-28408748
Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disease, and bacterial infection plays a role in its pathogenesis. Bacteria secrete nanometer-sized extracellular vesicles (EVs), which may induce more immune dysfunction and inflammation than the bacteria themselves. We hypothesized that the microbiome of lung EVs might have distinct characteristics depending on the presence of COPD and smoking status. We analyzed and compared the microbiomes of 13 nonsmokers with normal spirometry, 13 smokers with normal spirometry (healthy smokers) and 13 patients with COPD by using 16S ribosomal RNA gene sequencing of surgical lung tissue and lung EVs. Subjects were matched for age and sex in all groups and for smoking levels in the COPD and healthy smoker groups. Each group included 12 men and 1 woman with the same mean age of 65.5 years. In all groups, EVs consistently showed more operational taxonomic units (OTUs) than lung tissue. In the healthy smoker and COPD groups, EVs had a higher Shannon index and a lower Simpson index than lung tissue and this trend was more prominent in the COPD group. Principal component analysis (PCA) showed clusters based on sample type rather than participants' clinical characteristics. Stenotrophomonas, Propionibacterium and Alicyclobacillus were the most commonly found genera. Firmicutes were highly present in the EVs of the COPD group compared with other samples or groups. Our analysis of the lung microbiome revealed that the bacterial communities present in the EVs and in the COPD group possessed distinct characteristics with differences in the OTUs, diversity indexes and PCA clustering.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fumar / Enfermedad Pulmonar Obstructiva Crónica / Microbiota / Vesículas Extracelulares / Pulmón Tipo de estudio: Observational_studies Límite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Exp Mol Med Asunto de la revista: BIOLOGIA MOLECULAR / BIOQUIMICA Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fumar / Enfermedad Pulmonar Obstructiva Crónica / Microbiota / Vesículas Extracelulares / Pulmón Tipo de estudio: Observational_studies Límite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Exp Mol Med Asunto de la revista: BIOLOGIA MOLECULAR / BIOQUIMICA Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos