Regulation of miR-21 expression in human melanoma via UV-ray-induced melanin pigmentation.
Environ Toxicol
; 32(8): 2064-2069, 2017 Aug.
Article
en En
| MEDLINE
| ID: mdl-28398611
Excessive environmental ultraviolet (UV) radiation produces genetic mutations that can lead to skin cancer. This study was designed to assess the potential inhibitory activity of microRNA-21 (miR-21) on the UV irradiation-stimulated melanogenesis signal pathway in melanoma cells. The molecular mechanism of miR-21-induced inhibitory activity on UV-ray-stimulated melanogenesis-regulating proteins was examined in A375.S2 human melanoma and B16F10 mouse melanoma cells. UV irradiation for 30 min induced melanogenesis signal pathway by increasing melanin production and the number of A375.S2 cells. Similarly, UV radiation increased the expression of α-melanocyte-stimulating hormone (α-MSH) protein and decreased the melanogenesis-regulating signal, such as EGFR and Akt phosphorylation. Notably, miR-21 overexpression in UV-ray-stimulated A375.S2 cells decreased α-MSH expression and increased EGFR and Akt phosphorylation levels. Furthermore, miR-21 on UV-ray- induced melanogenesis was down-regulated by the Akt inhibitor and the EGFR inhibitor (Gefitinib). Results suggest that the suppressive activity of miR-21 on UV-ray-stimulated melanogenesis may involve the down-regulation of α-MSH and the activation in both of EGFR and Akt.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Cutáneas
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Rayos Ultravioleta
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MicroARNs
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Melaninas
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Melanoma
Límite:
Animals
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Humans
Idioma:
En
Revista:
Environ Toxicol
Asunto de la revista:
SAUDE AMBIENTAL
/
TOXICOLOGIA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Taiwán
Pais de publicación:
Estados Unidos