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Molecular Insight into Evolution of Symbiosis between Breast-Fed Infants and a Member of the Human Gut Microbiome Bifidobacterium longum.
Yamada, Chihaya; Gotoh, Aina; Sakanaka, Mikiyasu; Hattie, Mitchell; Stubbs, Keith A; Katayama-Ikegami, Ayako; Hirose, Junko; Kurihara, Shin; Arakawa, Takatoshi; Kitaoka, Motomitsu; Okuda, Shujiro; Katayama, Takane; Fushinobu, Shinya.
Afiliación
  • Yamada C; Department of Biotechnology, The University of Tokyo, Tokyo 113-8657, Japan; Graduate School of Biostudies, Kyoto University, Kyoto 606-8052, Japan.
  • Gotoh A; Graduate School of Biostudies, Kyoto University, Kyoto 606-8052, Japan.
  • Sakanaka M; Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Ishikawa 921-8836, Japan.
  • Hattie M; School of Chemistry and Biochemistry, The University of Western Australia, Crawley, WA 6009, Australia.
  • Stubbs KA; School of Chemistry and Biochemistry, The University of Western Australia, Crawley, WA 6009, Australia.
  • Katayama-Ikegami A; Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Ishikawa 921-8836, Japan.
  • Hirose J; School of Human Cultures, The University of Shiga Prefecture, Hikone, Shiga 522-8533, Japan.
  • Kurihara S; Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Ishikawa 921-8836, Japan.
  • Arakawa T; Department of Biotechnology, The University of Tokyo, Tokyo 113-8657, Japan.
  • Kitaoka M; Food Research Institute, National Agriculture and Food Research Organization, Ibaraki 305-8642, Japan.
  • Okuda S; Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-8510, Japan.
  • Katayama T; Graduate School of Biostudies, Kyoto University, Kyoto 606-8052, Japan; Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Ishikawa 921-8836, Japan. Electronic address: takane@lif.kyoto-u.ac.jp.
  • Fushinobu S; Department of Biotechnology, The University of Tokyo, Tokyo 113-8657, Japan. Electronic address: asfushi@mail.ecc.u-tokyo.ac.jp.
Cell Chem Biol ; 24(4): 515-524.e5, 2017 Apr 20.
Article en En | MEDLINE | ID: mdl-28392148
Breast-fed infants generally have a bifidobacteria-rich microbiota with recent studies indicating that human milk oligosaccharides (HMOs) selectively promote bifidobacterial growth. Bifidobacterium bifidum possesses a glycoside hydrolase family 20 lacto-N-biosidase for liberating lacto-N-biose I from lacto-N-tetraose, an abundant HMO unique to human milk, while Bifidobacterium longum subsp. longum has a non-classified enzyme (LnbX). Here, we determined the crystal structure of the catalytic domain of LnbX and provide evidence for creation of a novel glycoside hydrolase family, GH136. The structure, in combination with inhibition and mutation studies, provides insight into the molecular mechanism and broader substrate specificity of this enzyme. Moreover, through genetic studies, we show that lnbX is indispensable for B. longum growth on lacto-N-tetraose and is a key genetic factor for persistence in the gut of breast-fed infants. Overall, this study reveals possible evolutionary routes for the emergence of symbiosis between humans and bifidobacterial species in the infant gut.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Evolución Molecular / Microbioma Gastrointestinal / Bifidobacterium longum / Leche Humana Límite: Humans / Infant Idioma: En Revista: Cell Chem Biol Año: 2017 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Evolución Molecular / Microbioma Gastrointestinal / Bifidobacterium longum / Leche Humana Límite: Humans / Infant Idioma: En Revista: Cell Chem Biol Año: 2017 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos