Your browser doesn't support javascript.
loading
Capillary blood collected on volumetric absorptive microsampling (VAMS) device for monitoring hydroxychloroquine in rheumatoid arthritis patients.
Qu, Ying; Brady, Kelley; Apilado, Robert; O'Malley, Tyler; Reddy, Smitha; Chitkara, Puja; Ibarra, Claudia; Alexander, Roberta Vezza; Dervieux, Thierry.
Afiliación
  • Qu Y; Research and Development, Exagen Diagnostics Inc., Vista, CA, USA. Electronic address: yqu@exagen.com.
  • Brady K; Research and Development, Exagen Diagnostics Inc., Vista, CA, USA.
  • Apilado R; Research and Development, Exagen Diagnostics Inc., Vista, CA, USA.
  • O'Malley T; Research and Development, Exagen Diagnostics Inc., Vista, CA, USA.
  • Reddy S; Arthritis Care and Research Center, Poway, CA, USA.
  • Chitkara P; Center for Arthritis and Rheumatologic Excellence, Chula Vista, CA, USA.
  • Ibarra C; Research and Development, Exagen Diagnostics Inc., Vista, CA, USA.
  • Alexander RV; Research and Development, Exagen Diagnostics Inc., Vista, CA, USA.
  • Dervieux T; Research and Development, Exagen Diagnostics Inc., Vista, CA, USA.
J Pharm Biomed Anal ; 140: 334-341, 2017 Jun 05.
Article en En | MEDLINE | ID: mdl-28391006
A novel technique for collection of capillary blood, termed volumetric absorptive microsampling (VAMS), has been recently cleared by the FDA for collection of human blood. VAMS absorbs a fixed volume of blood (10µl) and overcomes area bias and homogeneity issues associated with dried blood spot (DBS). This study is the application of VAMS for therapeutic drug monitoring (TDM) in human capillary blood. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) workflow for analysis of VAMS sample was developed and validated. Concentration of hydroxychloroquine (HCQ) and its metabolites, desethylhydroxychloroquine (DHCQ), desethylchloroquine (DCQ), and bisdesethylchloroquine (BDCQ), in capillary blood on VAMS sampler were compared to those in venous blood in rheumatoid arthritis patients. Feasibility of capillary blood collected on both VAMS and DBS card were evaluated on patients. Stability of dried capillary blood on VAMS was also examined. Our results established that VAMS is a simple and accurate sampling technique that delivers the benefits of DBS sampling while overcoming the issues associated with hematocrit and homogeneity. It requires a small blood volume, simplifies sample logistics management, and may allow sample collection in the patient's home setting.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Artritis Reumatoide Límite: Humans Idioma: En Revista: J Pharm Biomed Anal Año: 2017 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Artritis Reumatoide Límite: Humans Idioma: En Revista: J Pharm Biomed Anal Año: 2017 Tipo del documento: Article Pais de publicación: Reino Unido