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How the presence of a small molecule affects the complex coacervation between lactoferrin and ß-lactoglobulin.
Tavares, Guilherme M; Croguennec, Thomas; Hamon, Pascaline; Carvalho, Antônio F; Bouhallab, Saïd.
Afiliación
  • Tavares GM; STLO, UMR1253, INRA, Agrocampus Ouest, 35000 Rennes, France; Laboratory of Research in Milk Products, Universidade Federal de Viçosa, BR-36570 Viçosa, Brazil.
  • Croguennec T; STLO, UMR1253, INRA, Agrocampus Ouest, 35000 Rennes, France.
  • Hamon P; STLO, UMR1253, INRA, Agrocampus Ouest, 35000 Rennes, France.
  • Carvalho AF; Laboratory of Research in Milk Products, Universidade Federal de Viçosa, BR-36570 Viçosa, Brazil.
  • Bouhallab S; STLO, UMR1253, INRA, Agrocampus Ouest, 35000 Rennes, France. Electronic address: said.bouhallab@rennes.inra.fr.
Int J Biol Macromol ; 102: 192-199, 2017 Sep.
Article en En | MEDLINE | ID: mdl-28389403
Heteroprotein complex coacervation corresponds to the formation of two liquid phases in equilibrium induced by the interaction of two oppositely charged proteins. The more concentrated phase known as coacervate phase, has attracted interest from several fields of science due to its potential applications for example for encapsulation and delivery of bioactives. Prior such application, it is necessary to understand how the presence of small ligands affects the complex coacervation. In this work, we report on the interaction of small ligand with individual proteins ß-lactoglobulin (ß-LG) and lactoferrin (LF) and consequences on their complex coacervation. ANS (8-Anilinonaphthalene-1-sulfonic acid), a fluorescent probe, was used as model ligand. While ANS did not interact with ß-LG, it presented two sets of binding sites with LF inducing its self-aggregation. Depending on its concentration, ANS modulated the shape of ß-LG-LF macromolecular assembly. Coacervates were observed for ANS/LF molar ratio <25 against amorphous aggregates for higher ANS/LF molar ratios. A maximum loading capacity of around 40mg of ANS per gram of LF in the formed heteroprotein coacervates was reached.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Electricidad Estática / Lactoferrina / Lactoglobulinas / Naftalenosulfonatos de Anilina Límite: Animals Idioma: En Revista: Int J Biol Macromol Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Electricidad Estática / Lactoferrina / Lactoglobulinas / Naftalenosulfonatos de Anilina Límite: Animals Idioma: En Revista: Int J Biol Macromol Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos