Your browser doesn't support javascript.
loading
Cellular hydrogels based on pH-responsive chitosan-hydroxyapatite system.
Rogina, Anamarija; Ressler, Antonia; Matic, Igor; Gallego Ferrer, Gloria; Marijanovic, Inga; Ivankovic, Marica; Ivankovic, Hrvoje.
Afiliación
  • Rogina A; Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10001 Zagreb, Marulicev trg 19, p.p.177, Croatia. Electronic address: arogina@fkit.hr.
  • Ressler A; Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10001 Zagreb, Marulicev trg 19, p.p.177, Croatia. Electronic address: aressler@fkit.hr.
  • Matic I; Faculty of Science, University of Zagreb, HR-10001 Zagreb, Horvatovac102a, Croatia. Electronic address: igor.matic@biol.pmf.hr.
  • Gallego Ferrer G; Centro de Biomateriales e Ingeniería Tisular, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain; Biomedical Research Networking centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain. Electronic address: ggallego@ter.upv.es.
  • Marijanovic I; Faculty of Science, University of Zagreb, HR-10001 Zagreb, Horvatovac102a, Croatia. Electronic address: ingam@biol.pmf.hr.
  • Ivankovic M; Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10001 Zagreb, Marulicev trg 19, p.p.177, Croatia. Electronic address: mivank@fkit.hr.
  • Ivankovic H; Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10001 Zagreb, Marulicev trg 19, p.p.177, Croatia. Electronic address: hivan@fkit.hr.
Carbohydr Polym ; 166: 173-182, 2017 Jun 15.
Article en En | MEDLINE | ID: mdl-28385221
The development of bioactive injectable system as cell carrier with minimal impact on viability of encapsulated cells represents a great challenge. In the present work, we propose a new pH-responsive chitosan-hydroxyapatite-based hydrogel with sodium bicarbonate (NaHCO3) as the gelling agent. The in situ synthesis of hydroxyapatite phase has resulted in stable composite suspension and final homogeneous hydrogel. The application of sodium bicarbonate has allowed non-cytotoxic fast gelation of chitosan-hydroxyapatite within 4min, and without excess of sodium ions concentration. Rheological properties of crosslinked hydrogel have demonstrated possible behaviour as 'strong physical hydrogel'. The live dead staining has confirmed good viability and dispersion, as well as proliferation of encapsulated cells by the culture time. Presented preliminary results show good potential of chitosan-hydroxyapatite/NaHCO3 as a cell carrier, whose impact on the cell differentiation need to be confirmed by encapsulation of other cell phenotypes.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Carbohydr Polym Año: 2017 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Carbohydr Polym Año: 2017 Tipo del documento: Article Pais de publicación: Reino Unido