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Cryopreservation Of Nili-Ravi Buffalo Bull Sperm in Cryodiluent Supplemented with Lolium perenne Protein Preparations.
Qadeer, S; Khan, M A; Ansari, M S; Rakha, B A; Ejaz, R; Husna, A U; Azam, A; Ullah, N; Walker, V K; Akhter, S.
Afiliación
  • Qadeer S; Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
  • Khan MA; Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
  • Ansari MS; Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
  • Rakha BA; Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
  • Ejaz R; Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
  • Husna AU; Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
  • Azam A; Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
  • Ullah N; Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
  • Walker VK; Department of Biology, Queen's University, Kingston, Ontario, Canada.
  • Akhter S; Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan. shamim@uaar.edu.pk.
Cryo Letters ; 38(1): 43-50, 2017.
Article en En | MEDLINE | ID: mdl-28376139
BACKGROUND: Semen from the Nili-Ravi buffalo bull, Bubalus bubalis, shows poor survival after freeze storage compared to bovine (Bos taurus and Bos indicus) semen. Freeze-susceptibility distinctions in these two genera have been attributed to differences in sperm membranes. MATERIALS AND METHODS: We measured the impact of protein preparations derived from a frost-resistant perennial grass, Lolium perenne, with ice recrystallization inhibition activity on the low temperature storage of B. bubalis semen. RESULTS: When the L. perenne preparations (0.1, 1, 10 µg/mL) were added to buffalo semen [2 ejaculates per bull (N=3) per replicate (r=3)] in Tris-citrate extender (50×106sperm mL-1), there was no impact on semen quality, as measured by sperm motility and plasma membrane integrity, after storage at 4 degree C (P>0.05). However, when semen supplemented with the grass proteins (0.1 and 1 µg mL-1) was evaluated after freezing and storage in liquid nitrogen for 24 h, post-thaw sperm progressive motility and plasma membrane integrity was higher (P<0.05) than in control samples. Post-thaw sperm viability and sperm acrosome integrity was similar (P > 0.05) to controls. CONCLUSION: The improvement in cryopreserved buffalo sperm progressive motility and plasma membrane integrity suggests that the use of these easily-made preparations may improve fertility after cryopreservation and offers the prospect of improved conception rates after artificial insemination with cryopreservation.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación de Semen / Lolium / Extractos Vegetales / Criopreservación / Crioprotectores Límite: Animals Idioma: En Revista: Cryo Letters Asunto de la revista: BIOLOGIA / QUIMICA Año: 2017 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Reino Unido
Buscar en Google
Añadir a Mi BVS
Imprimir
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PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación de Semen / Lolium / Extractos Vegetales / Criopreservación / Crioprotectores Límite: Animals Idioma: En Revista: Cryo Letters Asunto de la revista: BIOLOGIA / QUIMICA Año: 2017 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Reino Unido