Effect of okadaic acid on phosphorylation-dephosphorylation of myosin light chain in aortic smooth muscle homogenate.
Biochem Biophys Res Commun
; 153(1): 156-61, 1988 May 31.
Article
en En
| MEDLINE
| ID: mdl-2837197
Myosin light chain phosphorylation in aortic smooth muscle homogenate reached a maximal level of 0.75 mol phosphate/mol light chain, and then declined. Addition of okadaic acid led to a sustained phosphorylation level of 1.7 mol/mol. In the absence of okadaic acid, phosphorylation was predominantly due to myosin light chain kinase, whereas in the presence of okadaic acid both myosin light chain kinase and protein kinase C were involved in phosphorylation. Okadaic acid inhibited dephosphorylation of the distinct sites in LC phosphorylated by either myosin light chain kinase or protein kinase C, suggesting that it exerts its effect through inhibition of myosin light chain phosphatases present in aortic homogenate.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Miosinas
/
Éteres Cíclicos
/
Músculo Liso Vascular
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1988
Tipo del documento:
Article
Pais de publicación:
Estados Unidos