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GHK-Cu-liposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis.
Wang, Xinying; Liu, Baoquan; Xu, Qiang; Sun, Haiyang; Shi, Meijun; Wang, Dan; Guo, Meihua; Yu, Jiawen; Zhao, Chunhui; Feng, Bin.
Afiliación
  • Wang X; Department of Biotechnology, Dalian Medical University, Dalian, China.
  • Liu B; Key Laboratory of Biotechnology and Bioresources Utilization, (Dalian Minzu University), Ministry of Education, Dalian, China.
  • Xu Q; Faculty of Chemical, Environmental and Biological Science and Technology, Dalian University of Technology, Dalian, China.
  • Sun H; Department of Biotechnology, Dalian Medical University, Dalian, China.
  • Shi M; Department of Biotechnology, Dalian Medical University, Dalian, China.
  • Wang D; Department of Biotechnology, Dalian Medical University, Dalian, China.
  • Guo M; Department of Biotechnology, Dalian Medical University, Dalian, China.
  • Yu J; Department of Biotechnology, Dalian Medical University, Dalian, China.
  • Zhao C; College of Life Sciences, Liaoning Normal University, Dalian, China.
  • Feng B; Department of Biotechnology, Dalian Medical University, Dalian, China.
Wound Repair Regen ; 25(2): 270-278, 2017 04.
Article en En | MEDLINE | ID: mdl-28370978
Glycyl-l-histidyl-l-lysine (GHK)-Cu is considered to be an activator of tissue remodeling, and has been used in cosmetic products. In this study, we prepared liposomes encapsulating GHK-Cu and analyzed their effect on human umbilical vein endothelial cells (HUVECs) proliferation and scald wound healing in mice. The nanoscaled GHK-Cu-liposomes promoted HUVECs proliferation, with a 33.1% increased rate. Flow cytometry analysis showed increased cell number at G1 stage and decreased cell number at G2 stage after GHK-Cu-liposomes treatment. Western blotting indicated that the expression of vascular endothelial growth factor and fibroblast grow factors-2 were both enhanced, as well as cell cycle-related proteins CDK4 and CyclinD1. In a mice scald model, angiogenesis in burned skin treated with GHK-Cu-liposomes was better compared with free GHK-Cu, and immunofluorescence analysis showed enhanced signal of CD31 and Ki67 in GHK-Cu-liposomes treated mice. Moreover, the wound healing time was shortened to 14 days post injury. Our results provide the evidence that GHK-Cu-liposomes could be utilized as a treatment for skin wounds.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligopéptidos / Cicatrización de Heridas / Quemaduras / Neovascularización Fisiológica / Proliferación Celular Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Wound Repair Regen Asunto de la revista: DERMATOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligopéptidos / Cicatrización de Heridas / Quemaduras / Neovascularización Fisiológica / Proliferación Celular Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Wound Repair Regen Asunto de la revista: DERMATOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos