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Insights into chitosan antibiofilm activity against methicillin-resistant Staphylococcus aureus.
Costa, E M; Silva, S; Tavaria, F K; Pintado, M M.
Afiliación
  • Costa EM; CBQF - Centro de Biotecnologia e Química Fina, Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Porto, Portugal.
  • Silva S; CBQF - Centro de Biotecnologia e Química Fina, Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Porto, Portugal.
  • Tavaria FK; CBQF - Centro de Biotecnologia e Química Fina, Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Porto, Portugal.
  • Pintado MM; CBQF - Centro de Biotecnologia e Química Fina, Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Porto, Portugal.
J Appl Microbiol ; 122(6): 1547-1557, 2017 Jun.
Article en En | MEDLINE | ID: mdl-28370752
AIMS: Chitosan is a natural compound that has been validated as a viable antimicrobial agent against Staphylococcus aureus. With this work we sought to evaluate the planktonic and sessile sensitivity of methicillin-resistant S. aureus to chitosan's activity and evaluate if methicillin-resistant S. aureus (MRSA) would be more or less sensitive to chitosan's activity than methicillin-sensitive S. aureus (MSSA). METHODS AND RESULTS: A group comprised of reference strains and clinical multiresistant isolates of MSSA and MRSA were used. Methicilin resistance effect upon chitosan activity was assessed in planktonic setting and in different phases of sessile colonization, namely adhesion, biofilm formation and mature biofilm through biomass and metabolism inhibition. The results obtained showed that S. aureus methicillin resistance mechanism did not impair chitosan's activity as the highest bacterial susceptibility was registered for MRSA. Chitosan was highly effective in inhibiting MSSA and MRSA strains in both planktonic and sessile settings with biofilm inhibition percentages reaching as high as 90% for MRSA. CONCLUSIONS: Staphylococcus aureus methicillin resistance did not impair chitosan's antimicrobial and antibiofilm activities and MRSA and MSSA were inhibited both in planktonic and sessile settings at low concentrations with great efficacy. SIGNIFICANCE AND IMPACT OF THE STUDY: Considering the obtained results chitosan shows potential as an alternative for the control of biofilm-related recalcitrant MRSA infections.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biopelículas / Quitosano / Staphylococcus aureus Resistente a Meticilina Límite: Humans Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biopelículas / Quitosano / Staphylococcus aureus Resistente a Meticilina Límite: Humans Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Reino Unido