Influenza virus immunosensor with an electro-active optical waveguide under potential modulation.
Opt Lett
; 42(7): 1205-1208, 2017 Apr 01.
Article
en En
| MEDLINE
| ID: mdl-28362730
Here we report the development of a novel immunosensor-based strategy for label-free detection of viral pathogens by incorporating a sandwich bioassay onto a single-mode, electro-active, integrated optical waveguide (EA-IOW). Our strategy begins with the functionalization of the electro-active waveguide surface with a capture antibody aimed at a specific virus antigen. Once the target antigen is bound to the photonic interface, it promotes the binding of a secondary antibody that has been labeled with a methylene blue (MB) dye. The MB is a redox-active probe whose optical absorption can be electrically modulated and interrogated with high sensitivity by a propagating waveguide mode. In this effort, we have targeted the hemagglutinin (HA) protein from the H5N1 avian influenza A virus to demonstrate the capabilities of the EA-IOW device for detection and quantification of an important antigen. Our initial results for the HA H5N1 influenza virus show a remarkable limit of detection in the pico-molar range.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Inmunoensayo
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Técnicas Biosensibles
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Electricidad
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Subtipo H5N1 del Virus de la Influenza A
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Dispositivos Ópticos
Idioma:
En
Revista:
Opt Lett
Año:
2017
Tipo del documento:
Article
Pais de publicación:
Estados Unidos