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Differential autophagic effects of vital dyes in retinal pigment epithelial ARPE-19 and photoreceptor 661W cells.
Sheu, Shwu-Jiuan; Chen, Jiunn-Liang; Bee, Youn-Shen; Chen, Yi-An; Lin, Shi-Han; Shu, Chih-Wen.
Afiliación
  • Sheu SJ; Department of Ophthalmology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
  • Chen JL; School of Medicine, National Yang-Ming University, Taipei, Taiwan.
  • Bee YS; Department of Ophthalmology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
  • Chen YA; School of Medicine, National Yang-Ming University, Taipei, Taiwan.
  • Lin SH; Department of Optometry, Chung Hwa University of Medical Technology, Tainan, Taiwan.
  • Shu CW; Department of Ophthalmology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
PLoS One ; 12(3): e0174736, 2017.
Article en En | MEDLINE | ID: mdl-28358857
Indocyanine green (ICG) and brilliant blue G (BBG) are commonly used vital dyes to remove internal limiting membrane (ILM) in vitreoretinal surgery. The vital dyes have shown cytotoxic effects in ocular cells. Autophagy is a stress responsive pathway for either protecting cells or promoting cell death. However, the role of autophagy in ocular cells in response to the vital dyes remains unknown. In this study, we found that ICG and BBG reduced cell viability in both human retinal pigment epithelial ARPE-19 and mouse photoreceptor 661W cells. ICG and BBG induced lipidated GFP-LC3-II and LC3-II in ARPE-19 and 661W cells. Combination treatment with the autophagy inhibitor chloroquine indicated that ICG and BBG reduced autophagic flux in ARPE-19 cells, whereas the vital dyes induced autophagic flux in 661W cells. Moreover, genetic and pharmacological ablation of autophagy enhanced vital dyes-induced cytotoxicity in ocular cells. Dietary supplements, including resveratrol, lutein, and CoQ10, induced autophagy and diminished the cytotoxic effects of ICG and BBG in ocular cells. These results suggest that autophagy may protect ARPE-19 and 661W cells from vital dyes-induced damage.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colorantes de Rosanilina / Autofagia / Colorantes / Epitelio Pigmentado de la Retina / Verde de Indocianina Límite: Animals / Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2017 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colorantes de Rosanilina / Autofagia / Colorantes / Epitelio Pigmentado de la Retina / Verde de Indocianina Límite: Animals / Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2017 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos