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Autophagy extends lifespan via vacuolar acidification.
Ruckenstuhl, Christoph; Netzberger, Christine; Entfellner, Iryna; Carmona-Gutierrez, Didac; Kickenweiz, Thomas; Stekovic, Slaven; Gleixner, Christina; Schmid, Christian; Klug, Lisa; Hajnal, Ivan; Sorgo, Alice G; Eisenberg, Tobias; Büttner, Sabrina; Marin O, Guillermo; Koziel, Rafael; Magnes, Christoph; Sinner, Frank; Pieber, Thomas R; Jansen-Dürr, Pidder; Fröhlich, Kai-Uwe; Kroemer, Guido; Madeo, Frank.
Afiliación
  • Ruckenstuhl C; Institute for Molecular Biosciences, University of Graz, 8010 Graz, Austria.
  • Netzberger C; Institute for Molecular Biosciences, University of Graz, 8010 Graz, Austria.
  • Entfellner I; Institute for Molecular Biosciences, University of Graz, 8010 Graz, Austria.
  • Carmona-Gutierrez D; Institute for Molecular Biosciences, University of Graz, 8010 Graz, Austria.
  • Kickenweiz T; Institute for Molecular Biosciences, University of Graz, 8010 Graz, Austria.
  • Stekovic S; Institute for Molecular Biosciences, University of Graz, 8010 Graz, Austria.
  • Gleixner C; Institute for Molecular Biosciences, University of Graz, 8010 Graz, Austria.
  • Schmid C; Institute for Molecular Biosciences, University of Graz, 8010 Graz, Austria.
  • Klug L; Institute for Molecular Biosciences, University of Graz, 8010 Graz, Austria.
  • Hajnal I; Institute for Molecular Biosciences, University of Graz, 8010 Graz, Austria.
  • Sorgo AG; Institute for Molecular Biosciences, University of Graz, 8010 Graz, Austria.
  • Eisenberg T; Institute for Molecular Biosciences, University of Graz, 8010 Graz, Austria.
  • Büttner S; Institute for Molecular Biosciences, University of Graz, 8010 Graz, Austria.
  • Marin O G; INSERM, U848, F-94805 Villejuif, France. ; Institut Gustave Roussy, F-94805 Villejuif, France.; Université Paris Sud, Paris 11, F-94805 Villejuif, France.
  • Koziel R; Institute for Biomedical Aging Research (IBA), Austrian Academy of Sciences, 6020 Innsbruck, Austria.
  • Magnes C; HEALTH-Institute for Biomedicine and Health Sciences, Joanneum Research Forschungsgesellschaft m.b.H., 8010 Graz, Austria.
  • Sinner F; HEALTH-Institute for Biomedicine and Health Sciences, Joanneum Research Forschungsgesellschaft m.b.H., 8010 Graz, Austria.; Division of Endocrinology and Metabolism, Department of Internal Medicine, Medical University of Graz, 8036 Graz, Austria.
  • Pieber TR; HEALTH-Institute for Biomedicine and Health Sciences, Joanneum Research Forschungsgesellschaft m.b.H., 8010 Graz, Austria.; Division of Endocrinology and Metabolism, Department of Internal Medicine, Medical University of Graz, 8036 Graz, Austria.
  • Jansen-Dürr P; Institute for Biomedical Aging Research (IBA), Austrian Academy of Sciences, 6020 Innsbruck, Austria.
  • Fröhlich KU; Institute for Molecular Biosciences, University of Graz, 8010 Graz, Austria.
  • Kroemer G; INSERM, U848, F-94805 Villejuif, France. ; Institut Gustave Roussy, F-94805 Villejuif, France.; Metabolomics Platform, Institut Gustave Roussy, F-94805 Villejuif, France.; Centre de Recherche des Cordeliers, F-75005 Paris, France.; Pôle de Biologie, Hôpital Européen Georges Pompidou, AP-HP, F-75908
  • Madeo F; Institute for Molecular Biosciences, University of Graz, 8010 Graz, Austria.
Microb Cell ; 1(5): 160-162, 2014 May 05.
Article en En | MEDLINE | ID: mdl-28357240
Methionine restriction (MetR) is one of the rare regimes that prolongs lifespan across species barriers. Using a yeast model, we recently demonstrated that this lifespan extension is promoted by autophagy, which in turn requires vacuolar acidification. Our study is the first to place autophagy as one of the major players required for MetR-mediated longevity. In addition, our work identifies vacuolar acidification as a key downstream element of autophagy induction under MetR, and possibly after rapamycin treatment. Unlike other amino acids, methionine plays pleiotropic roles in many metabolism-relevant pathways. For instance, methionine (i) is the N-terminal amino acid of every newly translated protein; (ii) acts as the central donor of methyl groups through S-adenosyl methionine (SAM) during methylation reactions of proteins, DNA or RNA; and (iii) provides the sulfhydryl groups for FeS-cluster formation and redox detoxification via transsulfuration to cysteine. Intriguingly, MetR causes lifespan extension, both in yeast and in rodents. We could show that in Saccharomyces cerevisiae, chronological lifespan (CLS) is increased in two specific methionine-auxotrophic strains (namely Δmet2 and Δmet15).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Microb Cell Año: 2014 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Austria

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Microb Cell Año: 2014 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Austria