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Targeted Diazotransfer Reagents Enable Selective Modification of Proteins with Azides.
Lohse, Jonas; Swier, Lotteke J Y M; Oudshoorn, Ruben C; Médard, Guillaume; Kuster, Bernhard; Slotboom, Dirk-Jan; Witte, Martin D.
Afiliación
  • Lohse J; Chemical Biology II, Stratingh Institute for Chemistry, University of Groningen , Nijenborgh 7, 9747 AG Groningen, The Netherlands.
  • Swier LJYM; Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute , Nijenborgh 4, 9747 AG Groningen, The Netherlands.
  • Oudshoorn RC; Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute , Nijenborgh 4, 9747 AG Groningen, The Netherlands.
  • Médard G; Chair for Proteomics and Bioanalytics, WZW, Technical University of Munich , Emil-Erlenmeyer-Forum 5, 85354 Freising, Germany.
  • Kuster B; Chair for Proteomics and Bioanalytics, WZW, Technical University of Munich , Emil-Erlenmeyer-Forum 5, 85354 Freising, Germany.
  • Slotboom DJ; Center for Integrated Protein Science Munich (CIPSM) , Butenandtstraße 5, 81377 Munich, Germany.
  • Witte MD; Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute , Nijenborgh 4, 9747 AG Groningen, The Netherlands.
Bioconjug Chem ; 28(4): 913-917, 2017 04 19.
Article en En | MEDLINE | ID: mdl-28355874
In chemical biology, azides are used to chemically manipulate target structures in a bioorthogonal manner for a plethora of applications ranging from target identification to the synthesis of homogeneously modified protein conjugates. While a variety of methods have been established to introduce the azido group into recombinant proteins, a method that directly converts specific amino groups in endogenous proteins is lacking. Here, we report the first biotin-tethered diazotransfer reagent DtBio and demonstrate that it selectively modifies the model proteins streptavidin and avidin and the membrane protein BioY on cell surface. The reagent converts amines in the proximity of the binding pocket to azides and leaves the remaining amino groups in streptavidin untouched. Reagents of this novel class will find use in target identification as well as the selective functionalization and bioorthogonal protection of proteins.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Azidas / Proteínas Bacterianas / Biotina / Avidina / Lactococcus lactis / Estreptavidina / Escherichia coli Idioma: En Revista: Bioconjug Chem Asunto de la revista: BIOQUIMICA Año: 2017 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Azidas / Proteínas Bacterianas / Biotina / Avidina / Lactococcus lactis / Estreptavidina / Escherichia coli Idioma: En Revista: Bioconjug Chem Asunto de la revista: BIOQUIMICA Año: 2017 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos