Preliminary characterization of a mutant of herpes simplex virus type 1 selected for acycloguanosine resistance in vitro.
J Med Virol
; 24(3): 251-62, 1988 Mar.
Article
en En
| MEDLINE
| ID: mdl-2835423
In this paper we report on the preliminary characterization of a mutant of herpes simplex virus type 1 (HSV-1) selected for acycloguanosine (acyclovir, ACV) resistance in vitro. The ACVr virus was examined for a series of parameters that include chemosensitivity assay, thymidine kinase (TK) activity, in vitro and in vivo growth, and mutation mapping. The data obtained indicate that a mutated TK gene is responsible for the ACVr phenotype. A distinctive feature of this mutant is the high level of resistance exhibited to ACV (100 microM) and the concomitant presence of a functional TK activity. Such a property makes this virus useful as a model for the study of viral resistance to nucleoside-type analogues in HSV.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Aciclovir
/
Simplexvirus
Límite:
Animals
Idioma:
En
Revista:
J Med Virol
Año:
1988
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
Estados Unidos