Development of an Assay for Measuring Proprotein-Conversion Activity on a Multiplex Magnetic Bead-Based Array Platform.
Adv Exp Med Biol
; 974: 321-326, 2017.
Article
en En
| MEDLINE
| ID: mdl-28353252
This chapter describes a protocol for measuring prolyl oligopeptidase (POP) activity using a biotinylated peptide substrate coupled to magnetic microspheres. The complex is incubated in the presence of a pituitary extract and activity can be detected by loss of the biotin label. The assay can be multiplexed for measuring multiple proprotein-cleaving enzymes simultaneously and can be used in analyses of neuropsychiatric diseases in which proteolytic cleavage of biologically active peptides is known to play a role.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Hipófisis
/
Serina Endopeptidasas
/
Separación Inmunomagnética
/
Proteínas del Tejido Nervioso
Límite:
Humans
Idioma:
En
Revista:
Adv Exp Med Biol
Año:
2017
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Estados Unidos