Your browser doesn't support javascript.
loading
Decreased Glutamatergic Activity in the Frontal Cortex of Single Prolonged Stress Model: In vivo and Ex Vivo Proton MR Spectroscopy.
Lim, Song-I; Song, Kyu-Ho; Yoo, Chi-Hyeon; Woo, Dong-Cheol; Choe, Bo-Young.
Afiliación
  • Lim SI; Department of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
  • Song KH; Research Institute of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
  • Yoo CH; Asan Institute for Life Sciences, Asan Medical Center, Seoul, Republic of Korea.
  • Woo DC; Department of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
  • Choe BY; Research Institute of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
Neurochem Res ; 42(8): 2218-2229, 2017 Aug.
Article en En | MEDLINE | ID: mdl-28349360
Single prolonged stress (SPS) is one of the preclinical models of posttraumatic stress disorder (PTSD) in humans. Not every traumatized person develops PTSD and the onset of the disease varies from months to many years after exposure to life-threatening events. The pathogenetic neurometabolites in PTSD have not been investigated to date, and could provide a means for therapeutic interventions. Therefore the present study aimed to evaluate neurochemical changes in the frontal cortex in the SPS model during time-dependent sensitization using in vivo and ex vivo proton magnetic spectroscopy (1H-MRS). Twenty-one male Sprague-Dawley rats (200-220 g) were randomly assigned into two groups (Control, n = 10; SPS, n = 11). SPS consists of three consecutive stressors (restraint, forced swimming, and ether exposure) followed by 7 days without disturbance. In vivo 1H-MRS scans were conducted at baseline, immediately after SPS, and 3 and 7 days after SPS to quantify time-dependent alterations in the frontal cortex. On day 7, all animals were sacrificed and ex vivo 1H-MRS was performed. After SPS exposure, the SPS group showed signs of excitatory activities (glutamate) and cellular membrane turnover (choline and total choline) for 7 days. After the time-sensitization period, the SPS group showed lower glutamate and creatine levels and higher choline and lactate levels than the control group. These results indicate that SPS induces sustained adaptation of glutamatergic neuronal activity in the frontal cortex. Therefore, we conclude that SPS-induced stress reduces glutamatergic metabolism in the frontal cortex.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Psicológico / Ácido Glutámico / Modelos Animales de Enfermedad / Espectroscopía de Protones por Resonancia Magnética / Lóbulo Frontal Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Neurochem Res Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Psicológico / Ácido Glutámico / Modelos Animales de Enfermedad / Espectroscopía de Protones por Resonancia Magnética / Lóbulo Frontal Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Neurochem Res Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos