Your browser doesn't support javascript.
loading
Sub-additive effects of photodynamic therapy combined with erlotinib for the treatment of epidermoid carcinoma: An in vitro study.
Gontijo, Sávio M L; Felizali, Renata C; Guimarães, Pedro P G; Santos, Robson A S; Sinisterra, Rubén D; Cortés, Maria E; Araújo, Patrícia V.
Afiliación
  • Gontijo SML; Restorative Dentistry Department, Faculty of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, MG, Brazil.
  • Felizali RC; Restorative Dentistry Department, Faculty of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, MG, Brazil.
  • Guimarães PPG; Chemistry Department, Institute of Exact Sciences, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, MG, Brazil.
  • Santos RAS; Department of Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, MG, Brazil.
  • Sinisterra RD; Chemistry Department, Institute of Exact Sciences, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, MG, Brazil.
  • Cortés ME; Restorative Dentistry Department, Faculty of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, MG, Brazil.
  • Araújo PV; Restorative Dentistry Department, Faculty of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, MG, Brazil. Electronic address: patriciavalenteufmg@gmail.com.
Photodiagnosis Photodyn Ther ; 18: 252-256, 2017 Jun.
Article en En | MEDLINE | ID: mdl-28344047
BACKGROUND: Photodynamic therapy (PDT) is an antitumour treatment that employs the combination of a photosensitive compound, oxygen and visible light. To improve the antitumour activity of PDT, the present study used the strategy of combining PDT with erlotinib (ERL), a drug frequently used in the treatment of epidermoid carcinoma. METHODS: An MTT cell viability assay was used to evaluate the cytotoxicity of PDT combined with ERL on A431 epidermoid carcinoma cells in vitro. This study evaluated the cytotoxicity of the following treatments: red laser irradiation (660nm) at different power densities (1.25-180J/cm2), the photosensitizer methylene blue (MB) at concentrations of 0.39-100µM, PDT (12.5µM MB and laser power densities from 1.25 to 180J/cm2), and PDT (12.5µM MB and a laser density of 120J/cm2) plus ERL (1µM). RESULTS: The laser power densities that were tested showed no cytotoxicity in A431 cells. MB showed a dose-dependent cytotoxicity. In PDT, an increase in the dose of light resulted in an increase in the cytotoxicity of MB. In addition, there was a sub-additive effect between PDT and ERL compared to the effect of each therapy alone. CONCLUSIONS: The sub-additive effect between PDT and ERL suggests that their combination may be an important strategy in the treatment of epidermoid carcinoma.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fotoquimioterapia / Carcinoma de Células Escamosas / Quimioradioterapia / Clorhidrato de Erlotinib Límite: Humans Idioma: En Revista: Photodiagnosis Photodyn Ther Asunto de la revista: DIAGNOSTICO POR IMAGEM / TERAPEUTICA Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fotoquimioterapia / Carcinoma de Células Escamosas / Quimioradioterapia / Clorhidrato de Erlotinib Límite: Humans Idioma: En Revista: Photodiagnosis Photodyn Ther Asunto de la revista: DIAGNOSTICO POR IMAGEM / TERAPEUTICA Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos