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SUMOylation Inhibition Mediated by Disruption of SUMO E1-E2 Interactions Confers Plant Susceptibility to Necrotrophic Fungal Pathogens.
Castaño-Miquel, Laura; Mas, Abraham; Teixeira, Inês; Seguí, Josep; Perearnau, Anna; Thampi, Bhagyasree N; Schapire, Arnaldo L; Rodrigo, Natalia; La Verde, Gaelle; Manrique, Silvia; Coca, Maria; Lois, L Maria.
Afiliación
  • Castaño-Miquel L; Center for Research in Agricultural Genomics - CRAG, Edifici CRAG-Campus UAB, Bellaterra (Cerdanyola del Vallés), 08193 Barcelona, Spain.
  • Mas A; Center for Research in Agricultural Genomics - CRAG, Edifici CRAG-Campus UAB, Bellaterra (Cerdanyola del Vallés), 08193 Barcelona, Spain.
  • Teixeira I; Center for Research in Agricultural Genomics - CRAG, Edifici CRAG-Campus UAB, Bellaterra (Cerdanyola del Vallés), 08193 Barcelona, Spain.
  • Seguí J; Center for Research in Agricultural Genomics - CRAG, Edifici CRAG-Campus UAB, Bellaterra (Cerdanyola del Vallés), 08193 Barcelona, Spain.
  • Perearnau A; Center for Research in Agricultural Genomics - CRAG, Edifici CRAG-Campus UAB, Bellaterra (Cerdanyola del Vallés), 08193 Barcelona, Spain.
  • Thampi BN; Center for Research in Agricultural Genomics - CRAG, Edifici CRAG-Campus UAB, Bellaterra (Cerdanyola del Vallés), 08193 Barcelona, Spain.
  • Schapire AL; Center for Research in Agricultural Genomics - CRAG, Edifici CRAG-Campus UAB, Bellaterra (Cerdanyola del Vallés), 08193 Barcelona, Spain.
  • Rodrigo N; Center for Research in Agricultural Genomics - CRAG, Edifici CRAG-Campus UAB, Bellaterra (Cerdanyola del Vallés), 08193 Barcelona, Spain.
  • La Verde G; Center for Research in Agricultural Genomics - CRAG, Edifici CRAG-Campus UAB, Bellaterra (Cerdanyola del Vallés), 08193 Barcelona, Spain.
  • Manrique S; Center for Research in Agricultural Genomics - CRAG, Edifici CRAG-Campus UAB, Bellaterra (Cerdanyola del Vallés), 08193 Barcelona, Spain.
  • Coca M; Center for Research in Agricultural Genomics - CRAG, Edifici CRAG-Campus UAB, Bellaterra (Cerdanyola del Vallés), 08193 Barcelona, Spain.
  • Lois LM; Center for Research in Agricultural Genomics - CRAG, Edifici CRAG-Campus UAB, Bellaterra (Cerdanyola del Vallés), 08193 Barcelona, Spain. Electronic address: maria.lois@cragenomica.es.
Mol Plant ; 10(5): 709-720, 2017 05 01.
Article en En | MEDLINE | ID: mdl-28343913
Protein modification by SUMO modulates essential biological processes in eukaryotes. SUMOylation is facilitated by sequential action of the E1-activating, E2-conjugating, and E3-ligase enzymes. In plants, SUMO regulates plant development and stress responses, which are key determinants in agricultural productivity. To generate additional tools for advancing our knowledge about the SUMO biology, we have developed a strategy for inhibiting in vivo SUMO conjugation based on disruption of SUMO E1-E2 interactions through expression of E1 SAE2UFDCt domain. Targeted mutagenesis and phylogenetic analyses revealed that this inhibition involves a short motif in SAE2UFDCt highly divergent across kingdoms. Transgenic plants expressing the SAE2UFDCt domain displayed dose-dependent inhibition of SUMO conjugation, and have revealed the existence of a post-transcriptional mechanism that regulates SUMO E2 conjugating enzyme levels. Interestingly, these transgenic plants displayed increased susceptibility to necrotrophic fungal infections by Botrytis cinerea and Plectosphaerella cucumerina. Early after fungal inoculation, host SUMO conjugation was post-transcriptionally downregulated, suggesting that targeting SUMOylation machinery could constitute a novel mechanism for fungal pathogenicity. These findings support the role of SUMOylation as a mechanism involved in plant protection from environmental stresses. In addition, the strategy for inhibiting SUMO conjugation in vivo described in this study might be applicable in important crop plants and other non-plant organisms regardless of their genetic complexity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Arabidopsis / Proteínas de Arabidopsis / Proteínas Modificadoras Pequeñas Relacionadas con Ubiquitina Idioma: En Revista: Mol Plant Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2017 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Arabidopsis / Proteínas de Arabidopsis / Proteínas Modificadoras Pequeñas Relacionadas con Ubiquitina Idioma: En Revista: Mol Plant Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2017 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido