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An aromatic amino acid within intracellular loop 2 of the prostaglandin EP2 receptor is a prerequisite for selective association and activation of Gαs.
Yano, Akiko; Takahashi, Yuko; Moriguchi, Hiromi; Inazumi, Tomoaki; Koga, Tomoaki; Otaka, Akira; Sugimoto, Yukihiko.
Afiliación
  • Yano A; Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
  • Takahashi Y; Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
  • Moriguchi H; Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
  • Inazumi T; Department of Pharmaceutical Biochemistry, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan; AMED-CREST, Tokyo 100-0004, Japan.
  • Koga T; Priority Organization for Innovation and Excellence, Kumamoto University, Kumamoto, Japan.
  • Otaka A; Department of Bioorganic Synthetic Chemistry, Graduate School of Pharmaceutical Sciences, Tokushima University, Tokushima, Japan.
  • Sugimoto Y; Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan; Department of Pharmaceutical Biochemistry, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan; AMED-CREST, Tokyo 100-0004, Japan. Electronic address: ysugi
Biochim Biophys Acta Mol Cell Biol Lipids ; 1862(6): 615-622, 2017 Jun.
Article en En | MEDLINE | ID: mdl-28336329
We previously demonstrated that the aromatic moiety of Tyr143 within the intracellular loop 2 (ICL2) region of the prostaglandin EP2 receptor plays a crucial role in Gs coupling. Here we investigated whether the ICL2 of the EP2 receptor directly binds to Gαs and whether an aromatic moiety affects this interaction. In Chinese hamster ovary cells, mutations of Tyr143 reduced the ability of the EP2 receptor to interact with G proteins as demonstrated by GTPγS sensitivity, as well as the ability of agonist-induced cAMP formation, with the rank order of Phe>Tyr (wild-type)=Trp>Leu>Ala (=0). We found that the wild-type ICL2 peptide (i2Y) and its mutant with Phe at Tyr143 (i2F) inhibited receptor-G protein complex formation of wild-type EP2 in membranes, whereas the Ala-substituted mutant (i2A) did not. Specific interactions between these peptides and the Gαs protein were detected by surface plasmon resonance, but Gαs showed different association rates, with a rank order of i2F>i2Y≫i2A, with similar dissociation rates. Moreover, i2F and i2Y, but not i2A activated membrane adenylyl cyclase. These results indicate that the ICL2 region of the EP2 receptor is its potential interaction site with Gαs, and that the aromatic side chain moiety at position 143 is a determinant for the accessibility of the ICL2 to the Gαs protein.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromograninas / Subunidades alfa de la Proteína de Unión al GTP Gs / Subtipo EP2 de Receptores de Prostaglandina E Tipo de estudio: Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: Biochim Biophys Acta Mol Cell Biol Lipids Año: 2017 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromograninas / Subunidades alfa de la Proteína de Unión al GTP Gs / Subtipo EP2 de Receptores de Prostaglandina E Tipo de estudio: Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: Biochim Biophys Acta Mol Cell Biol Lipids Año: 2017 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Países Bajos