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DNA-mediated association of two histone-bound complexes of yeast Chromatin Assembly Factor-1 (CAF-1) drives tetrasome assembly in the wake of DNA replication.
Mattiroli, Francesca; Gu, Yajie; Yadav, Tejas; Balsbaugh, Jeremy L; Harris, Michael R; Findlay, Eileen S; Liu, Yang; Radebaugh, Catherine A; Stargell, Laurie A; Ahn, Natalie G; Whitehouse, Iestyn; Luger, Karolin.
Afiliación
  • Mattiroli F; Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, United States.
  • Gu Y; Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, United States.
  • Yadav T; Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, United States.
  • Balsbaugh JL; Weill Cornell Graduate School of Medical Sciences, New York, United States.
  • Harris MR; Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, United States.
  • Findlay ES; Department of Chemistry and Biochemistry, University of Colorado Boulder, Boulder, United States.
  • Liu Y; Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, United States.
  • Radebaugh CA; Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, United States.
  • Stargell LA; Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, United States.
  • Ahn NG; Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, United States.
  • Whitehouse I; Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, United States.
  • Luger K; Institute for Genome Architecture and Function, Colorado State University, Fort Collins, United States.
Elife ; 62017 03 18.
Article en En | MEDLINE | ID: mdl-28315523
Nucleosome assembly in the wake of DNA replication is a key process that regulates cell identity and survival. Chromatin assembly factor 1 (CAF-1) is a H3-H4 histone chaperone that associates with the replisome and orchestrates chromatin assembly following DNA synthesis. Little is known about the mechanism and structure of this key complex. Here we investigate the CAF-1•H3-H4 binding mode and the mechanism of nucleosome assembly. We show that yeast CAF-1 binding to a H3-H4 dimer activates the Cac1 winged helix domain interaction with DNA. This drives the formation of a transient CAF-1•histone•DNA intermediate containing two CAF-1 complexes, each associated with one H3-H4 dimer. Here, the (H3-H4)2 tetramer is formed and deposited onto DNA. Our work elucidates the molecular mechanism for histone deposition by CAF-1, a reaction that has remained elusive for other histone chaperones, and it advances our understanding of how nucleosomes and their epigenetic information are maintained through DNA replication.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ribonucleasas / Saccharomyces cerevisiae / ADN de Hongos / Histonas / Nucleosomas / Cromosomas Fúngicos / Proteínas de Saccharomyces cerevisiae / Replicación del ADN Tipo de estudio: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Elife Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ribonucleasas / Saccharomyces cerevisiae / ADN de Hongos / Histonas / Nucleosomas / Cromosomas Fúngicos / Proteínas de Saccharomyces cerevisiae / Replicación del ADN Tipo de estudio: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Elife Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido