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Lipoteichoic acids from Staphylococcus aureus stimulate proliferation of human non-small-cell lung cancer cells in vitro.
Hattar, Katja; Reinert, Christian P; Sibelius, Ulf; Gökyildirim, Mira Y; Subtil, Florentine S B; Wilhelm, Jochen; Eul, Bastian; Dahlem, Gabriele; Grimminger, Friedrich; Seeger, Werner; Grandel, Ulrich.
Afiliación
  • Hattar K; Department of Internal Medicine IV/V, University of Giessen and Marburg Lung Center (UGMLC), Klinikstrasse 33, Giessen, Germany.
  • Reinert CP; Department of Internal Medicine IV/V, University of Giessen and Marburg Lung Center (UGMLC), Klinikstrasse 33, Giessen, Germany.
  • Sibelius U; Department of Internal Medicine IV/V, University of Giessen and Marburg Lung Center (UGMLC), Klinikstrasse 33, Giessen, Germany.
  • Gökyildirim MY; Department of Internal Medicine IV/V, University of Giessen and Marburg Lung Center (UGMLC), Klinikstrasse 33, Giessen, Germany.
  • Subtil FSB; Department of Radiotherapy and Radiooncology, Philipps-University, Marburg, Germany.
  • Wilhelm J; Department of Internal Medicine II, University of Giessen and Marburg Lung Center (UGMLC), Giessen, Germany.
  • Eul B; Department of Internal Medicine IV/V, University of Giessen and Marburg Lung Center (UGMLC), Klinikstrasse 33, Giessen, Germany.
  • Dahlem G; Department of Internal Medicine IV/V, University of Giessen and Marburg Lung Center (UGMLC), Klinikstrasse 33, Giessen, Germany.
  • Grimminger F; Department of Internal Medicine IV/V, University of Giessen and Marburg Lung Center (UGMLC), Klinikstrasse 33, Giessen, Germany.
  • Seeger W; Department of Internal Medicine II, University of Giessen and Marburg Lung Center (UGMLC), Giessen, Germany.
  • Grandel U; Max-Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.
Cancer Immunol Immunother ; 66(6): 799-809, 2017 Jun.
Article en En | MEDLINE | ID: mdl-28314957
Pulmonary infections are frequent complications in lung cancer and may worsen its outcome and survival. Inflammatory mediators are suspected to promote tumor growth in non-small-cell lung cancer (NSCLC). Hence, bacterial pathogens may affect lung cancer growth by activation of inflammatory signalling. Against this background, we investigated the effect of purified lipoteichoic acids (LTA) of Staphylococcus aureus (S. aureus) on cellular proliferation and liberation of interleukin (IL)-8 in the NSCLC cell lines A549 and H226. A549 as well as H226 cells constitutively expressed TLR-2 mRNA. Even in low concentrations, LTA induced a prominent increase in cellular proliferation of A549 cells as quantified by automatic cell counting. In parallel, metabolic activity of A549 cells was enhanced. The increase in proliferation was accompanied by an increase in IL-8 mRNA expression and a dose- and time-dependent release of IL-8. Cellular proliferation as well as the release of IL-8 was dependent on specific ligation of TLR-2. Interestingly, targeting IL-8 by neutralizing antibodies completely abolished the LTA-induced proliferation of A549 cells. The pro-proliferative effect of LTA could also be reproduced in the squamous NSCLC cell line H226. In summary, LTA of S. aureus induced proliferation of NSCLC cell lines of adeno- and squamous cell carcinoma origin. Ligation of TLR-2 followed by auto- or paracrine signalling by endogenously synthesized IL-8 is centrally involved in LTA-induced tumor cell proliferation. Therefore, pulmonary infections may exert a direct pro-proliferative effect on lung cancer growth.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácidos Teicoicos / Carcinoma de Células Escamosas / Lipopolisacáridos / Interleucina-8 / Carcinoma de Pulmón de Células no Pequeñas / Proliferación Celular / Neoplasias Pulmonares Límite: Humans Idioma: En Revista: Cancer Immunol Immunother Asunto de la revista: ALERGIA E IMUNOLOGIA / NEOPLASIAS / TERAPEUTICA Año: 2017 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácidos Teicoicos / Carcinoma de Células Escamosas / Lipopolisacáridos / Interleucina-8 / Carcinoma de Pulmón de Células no Pequeñas / Proliferación Celular / Neoplasias Pulmonares Límite: Humans Idioma: En Revista: Cancer Immunol Immunother Asunto de la revista: ALERGIA E IMUNOLOGIA / NEOPLASIAS / TERAPEUTICA Año: 2017 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania