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A reconfigured Kennedy pathway which promotes efficient accumulation of medium-chain fatty acids in leaf oils.
Reynolds, Kyle B; Taylor, Matthew C; Cullerne, Darren P; Blanchard, Christopher L; Wood, Craig C; Singh, Surinder P; Petrie, James R.
Afiliación
  • Reynolds KB; Commonwealth Scientific and Industrial Research Organization, Agriculture and Food, Acton, ACT, Australia.
  • Taylor MC; Department of Primary Industries, Graham Centre for Agricultural Innovation, Charles Sturt University, Wagga Wagga, NSW, Australia.
  • Cullerne DP; ARC Industrial Transformation Training Centre for Functional Grains, Charles Sturt University, Wagga Wagga, NSW, Australia.
  • Blanchard CL; Commonwealth Scientific and Industrial Research Organization, Land and Water, Acton, ACT, Australia.
  • Wood CC; Commonwealth Scientific and Industrial Research Organization, Agriculture and Food, Acton, ACT, Australia.
  • Singh SP; School of Environmental and Life Sciences, University of Newcastle, Newcastle, NSW, Australia.
  • Petrie JR; ARC Industrial Transformation Training Centre for Functional Grains, Charles Sturt University, Wagga Wagga, NSW, Australia.
Plant Biotechnol J ; 15(11): 1397-1408, 2017 Nov.
Article en En | MEDLINE | ID: mdl-28301719
Medium-chain fatty acids (MCFA, C6-14 fatty acids) are an ideal feedstock for biodiesel and broader oleochemicals. In recent decades, several studies have used transgenic engineering to produce MCFA in seeds oils, although these modifications result in unbalance membrane lipid profiles that impair oil yields and agronomic performance. Given the ability to engineer nonseed organs to produce oils, we have previously demonstrated that MCFA profiles can be produced in leaves, but this also results in unbalanced membrane lipid profiles and undesirable chlorosis and cell death. Here we demonstrate that the introduction of a diacylglycerol acyltransferase from oil palm, EgDGAT1, was necessary to channel nascent MCFA directly into leaf oils and therefore bypassing MCFA residing in membrane lipids. This pathway resulted in increased flux towards MCFA rich leaf oils, reduced MCFA in leaf membrane lipids and, crucially, the alleviation of chlorosis. Deep sequencing of African oil palm (Elaeis guineensis) and coconut palm (Cocos nucifera) generated candidate genes of interest, which were then tested for their ability to improve oil accumulation. Thioesterases were explored for the production of lauric acid (C12:0) and myristic (C14:0). The thioesterases from Umbellularia californica and Cinnamomum camphora produced a total of 52% C12:0 and 40% C14:0, respectively, in transient leaf assays. This study demonstrated that the introduction of a complete acyl-CoA-dependent pathway for the synthesis of MFCA-rich oils avoided disturbing membrane homoeostasis and cell death phenotypes. This study outlines a transgenic strategy for the engineering of biomass crops with high levels of MCFA rich leaf oils.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aceites de Plantas / Hojas de la Planta / Arecaceae / Diacilglicerol O-Acetiltransferasa / Ácidos Grasos Idioma: En Revista: Plant Biotechnol J Asunto de la revista: BIOTECNOLOGIA / BOTANICA Año: 2017 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aceites de Plantas / Hojas de la Planta / Arecaceae / Diacilglicerol O-Acetiltransferasa / Ácidos Grasos Idioma: En Revista: Plant Biotechnol J Asunto de la revista: BIOTECNOLOGIA / BOTANICA Año: 2017 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido