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Contrasting antibody responses to intrasubtype superinfection with CRF02_AG.
Courtney, Colleen R; Mayr, Luzia; Nanfack, Aubin J; Banin, Andrew N; Tuen, Michael; Pan, Ruimin; Jiang, Xunqing; Kong, Xiang-Peng; Kirkpatrick, Allison R; Bruno, Daniel; Martens, Craig A; Sykora, Lydia; Porcella, Stephen F; Redd, Andrew D; Quinn, Thomas C; Nyambi, Phillipe N; Dürr, Ralf.
Afiliación
  • Courtney CR; Department of Microbiology, New York University School of Medicine, New York, NY, United States of America.
  • Mayr L; Department of Pathology, New York University School of Medicine, New York, NY, United States of America.
  • Nanfack AJ; Department of Pathology, New York University School of Medicine, New York, NY, United States of America.
  • Banin AN; Department of Pathology, New York University School of Medicine, New York, NY, United States of America.
  • Tuen M; Faculty of Medicine and Biomedical Sciences, University of Yaoundé, Yaoundé, Cameroon.
  • Pan R; Department of Pathology, New York University School of Medicine, New York, NY, United States of America.
  • Jiang X; Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY, United States of America.
  • Kong XP; Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY, United States of America.
  • Kirkpatrick AR; Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY, United States of America.
  • Bruno D; Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United States of America.
  • Martens CA; Genomics Unit, Research Technologies Branch, Rocky Mountain Laboratories, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States of America.
  • Sykora L; Genomics Unit, Research Technologies Branch, Rocky Mountain Laboratories, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States of America.
  • Porcella SF; Genomics Unit, Research Technologies Branch, Rocky Mountain Laboratories, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States of America.
  • Redd AD; Genomics Unit, Research Technologies Branch, Rocky Mountain Laboratories, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States of America.
  • Quinn TC; Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United States of America.
  • Nyambi PN; Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, United States of America.
  • Dürr R; Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United States of America.
PLoS One ; 12(3): e0173705, 2017.
Article en En | MEDLINE | ID: mdl-28288209
HIV superinfection describes the sequential infection of an individual with two or more unrelated HIV strains. Intersubtype superinfection has been shown to cause a broader and more potent heterologous neutralizing antibody response when compared to singly infected controls, yet the effects of intrasubtype superinfection remain controversial. Longitudinal samples were analyzed phylogenetically for pol and env regions using Next-Generation Sequencing and envelope cloning. The impact of CRF02_AG intrasubtype superinfection was assessed for heterologous neutralization and antibody binding responses. We compared two cases of CRF02_AG intrasubtype superinfection that revealed complete replacement of the initial virus by superinfecting CRF02_AG variants with signs of recombination. NYU6564, who became superinfected at an early time point, exhibited greater changes in antibody binding profiles and generated a more potent neutralizing antibody response post-superinfection compared to NYU6501. In contrast, superinfection occurred at a later time point in NYU6501 with strains harboring significantly longer V1V2 regions with no observable changes in neutralization patterns. Here we show that CRF02_AG intrasubtype superinfection can induce a cross-subtype neutralizing antibody response, and our data suggest timing and/or superinfecting viral envelope characteristics as contributing factors. These results highlight differential outcomes in intrasubtype superinfection and provide the first insight into cases with CRF02_AG, the fourth most prevalent HIV-1 strain worldwide.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sobreinfección / VIH-1 Límite: Female / Humans / Pregnancy Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sobreinfección / VIH-1 Límite: Female / Humans / Pregnancy Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos