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Progesterone increased ß-endorphin innervation of the locus coeruleus, but ovarian steroids had no effect on noradrenergic neurodegeneration.
Lima, Fernanda B; Leite, Cristiane M; Bethea, Cynthia L; Anselmo-Franci, Janete A.
Afiliación
  • Lima FB; Departamento de Ciências Fisiológicas, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil. Electronic address: fernanda.lima@ufsc.br.
  • Leite CM; Departamento de Morfologia, Fisiologia, e Patologia Básica, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, SP, Brazil. Electronic address: crismleite@yahoo.com.br.
  • Bethea CL; Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, OR, USA. Electronic address: betheac@ohsu.edu.
  • Anselmo-Franci JA; Departamento de Morfologia, Fisiologia, e Patologia Básica, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, SP, Brazil. Electronic address: jaafranc@usp.br.
Brain Res ; 1663: 1-8, 2017 05 15.
Article en En | MEDLINE | ID: mdl-28284896
With the decline of ovarian steroids levels at menopause, many women experience an increase in anxiety and stress sensitivity. The locus coeruleus (LC), a central source of noradrenaline (NE), is activated by stress and is inhibited by ß-endorphin. Moreover, increased NE has been implicated in pathological anxiety syndromes. Hormone replacement therapy (HRT) in menopause appears to decrease anxiety and vulnerability to stress. Therefore, we questioned the effect of HRT on the inhibitory ß-endorphin innervation of the LC. In addition, we found that progesterone protects serotoninergic neurons in monkeys, leading us to question whether ovarian steroids are also neuroprotective in LC neurons in monkeys. Adult Rhesus monkeys (Macaca mulatta) were ovariectomized, and either treated with Silastic capsules that contained estradiol, estradiol+progesterone, progesterone alone or that were empty (ovariectomized; control). After 1month, the LC was obtained and processed for immunohistochemistry for ß-endorphin and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL). The density of ß-endorphin axons was determined with image analysis using ImageJ. The TUNEL-positive neurons were counted in the entire LC. Progesterone-alone significantly increased the density of the ß-endorphin axons in the LC (p<0.01). No significant differences between groups in the number of TUNEL-positive cells in the LC were found. In conclusion, we found that HRT increases the inhibitory influence of ß-endorphin in the LC, which could, in turn, contribute to reduce anxiety and increase stress resilience. In addition, we did not find compelling evidence of neurodegeneration or neuroprotection by HRT in the LC of Rhesus monkeys.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Locus Coeruleus / Betaendorfina Límite: Animals Idioma: En Revista: Brain Res Año: 2017 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Locus Coeruleus / Betaendorfina Límite: Animals Idioma: En Revista: Brain Res Año: 2017 Tipo del documento: Article Pais de publicación: Países Bajos