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Pneumococcal nasopharyngeal carriage among children in Brazil prior to the introduction of the 10-valent conjugate vaccine: a culture- and PCR-based survey.
Rodrigues, H G; Pinto, T C A; Barros, R R; Teixeira, L M; Neves, F P G.
Afiliación
  • Rodrigues HG; Department of Microbiology and Parasitology,Universidade Federal Fluminense,Niterói, Rio de Janeiro,Brazil.
  • Pinto TCA; Department of Medical Microbiology,Universidade Federal do Rio de Janeiro,Rio de Janeiro,Brazil.
  • Barros RR; Department of Microbiology and Parasitology,Universidade Federal Fluminense,Niterói, Rio de Janeiro,Brazil.
  • Teixeira LM; Department of Medical Microbiology,Universidade Federal do Rio de Janeiro,Rio de Janeiro,Brazil.
  • Neves FPG; Department of Microbiology and Parasitology,Universidade Federal Fluminense,Niterói, Rio de Janeiro,Brazil.
Epidemiol Infect ; 145(8): 1720-1726, 2017 06.
Article en En | MEDLINE | ID: mdl-28264733
We performed two different approaches (broth enrichment step prior to culture (BEC) and PCR (BEPCR)) for detecting Streptococcus pneumoniae from nasopharyngeal specimens collected from 242 children aged <6 years attending one hospital (n = 140) and one childcare centre (n = 102) in a major urban area in Brazil. These specimens were collected immediately before the introduction of the 10-valent pneumococcal conjugate vaccine (PCV10) and the 13-valent vaccine (PCV13) for routine use in Brazil. Results were compared with previous findings obtained with direct culture (DC) on a selective medium. Colonisation prevalence was 58·3% (n = 141), being higher among children attending the childcare centre (62·7% vs. 55%). The culture-based methods (DC and BEC) enabled the detection of S. pneumoniae in 119 (49·2%) and 115 (47·5%) children, respectively. The PCR-based method (BEPCR) was more sensitive and 137 (56·6%) carriers were identified. Twenty-six serogroups/serotypes were identified, predominantly 6B, 19F, 14, 6A, 15C and 23F. Multiple colonisation was observed in 13 (5·4%) children. The estimated serotypes coverage of available PCVs was 40·4% for the 10-valent (included in the Brazilian immunisation programme) and 55·8% for the 13-valent (only available in private clinics). The use of robust approaches to obtain a more realistic insight about the asymptomatic carrier status is of paramount importance to estimate and assess the impact of vaccine implementation. The combination between culture-based and molecular methods constitutes a suitable strategy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Neumocócicas / Streptococcus pneumoniae / Recuento de Colonia Microbiana / Portador Sano / Nasofaringe / Reacción en Cadena de la Polimerasa Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Child, preschool / Female / Humans / Infant / Male / Newborn País/Región como asunto: America do sul / Brasil Idioma: En Revista: Epidemiol Infect Asunto de la revista: DOENCAS TRANSMISSIVEIS / EPIDEMIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Neumocócicas / Streptococcus pneumoniae / Recuento de Colonia Microbiana / Portador Sano / Nasofaringe / Reacción en Cadena de la Polimerasa Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Child, preschool / Female / Humans / Infant / Male / Newborn País/Región como asunto: America do sul / Brasil Idioma: En Revista: Epidemiol Infect Asunto de la revista: DOENCAS TRANSMISSIVEIS / EPIDEMIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido