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Diversity and genetic stability in banana genotypes in a breeding program using inter simple sequence repeats (ISSR) markers.
Silva, A V C; Nascimento, A L S; Vitória, M F; Rabbani, A R C; Soares, A N R; Lédo, A S.
Afiliación
  • Silva AV; Embrapa Tabuleiros Costeiros, Aracaju, SE, Brasil ana.veruska@embrapa.br.
  • Nascimento AL; Embrapa Tabuleiros Costeiros, Aracaju, SE, Brasil.
  • Vitória MF; Programa de Pós-Graduação em Agricultura e Biodiversidade, Universidade Federal de Sergipe, Aracaju, SE, Brasil.
  • Rabbani AR; Instituto Federal da Bahia, Porto Seguro, BA, Brasil.
  • Soares AN; Programa de Pós-Graduação em Agricultura e Biodiversidade, Universidade Federal de Sergipe, Aracaju, SE, Brasil.
  • Lédo AS; Embrapa Tabuleiros Costeiros, Aracaju, SE, Brasil.
Genet Mol Res ; 16(1)2017 Feb 23.
Article en En | MEDLINE | ID: mdl-28252170
Banana (Musa spp) is a fruit species frequently cultivated and consumed worldwide. Molecular markers are important for estimating genetic diversity in germplasm and between genotypes in breeding programs. The objective of this study was to analyze the genetic diversity of 21 banana genotypes (FHIA 23, PA42-44, Maçã, Pacovan Ken, Bucaneiro, YB42-47, Grand Naine, Tropical, FHIA 18, PA94-01, YB42-17, Enxerto, Japira, Pacovã, Prata-Anã, Maravilha, PV79-34, Caipira, Princesa, Garantida, and Thap Maeo), by using inter-simple sequence repeat (ISSR) markers. Material was generated from the banana breeding program of Embrapa Cassava & Fruits and evaluated at Embrapa Coastal Tablelands. The 12 primers used in this study generated 97.5% polymorphism. Four clusters were identified among the different genotypes studied, and the sum of the first two principal components was 48.91%. From the Unweighted Pair Group Method using Arithmetic averages (UPGMA) dendrogram, it was possible to identify two main clusters and subclusters. Two genotypes (Garantida and Thap Maeo) remained isolated from the others, both in the UPGMA clustering and in the principal cordinate analysis (PCoA). Using ISSR markers, we could analyze the genetic diversity of the studied material and state that these markers were efficient at detecting sufficient polymorphism to estimate the genetic variability in banana genotypes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Variación Genética / Repeticiones de Microsatélite / Musa / Fitomejoramiento Tipo de estudio: Prognostic_studies Idioma: En Revista: Genet Mol Res Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Variación Genética / Repeticiones de Microsatélite / Musa / Fitomejoramiento Tipo de estudio: Prognostic_studies Idioma: En Revista: Genet Mol Res Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil