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Highly Efficient Enzymatic Acylation of Dihydromyricetin by the Immobilized Lipase with Deep Eutectic Solvents as Cosolvent.
Cao, Shi-Lin; Deng, Xiao; Xu, Pei; Huang, Zi-Xuan; Zhou, Jian; Li, Xue-Hui; Zong, Min-Hua; Lou, Wen-Yong.
Afiliación
  • Cao SL; Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology , No. 381 Wushan Road, Guangzhou 510640, China.
  • Deng X; Department of Food Science, Foshan University , No. 18 Jiangwan Yi Road, Foshan 528000, China.
  • Xu P; Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology , No. 381 Wushan Road, Guangzhou 510640, China.
  • Huang ZX; Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology , No. 381 Wushan Road, Guangzhou 510640, China.
  • Zhou J; School of Chemistry and Chemical Engineering, South China University of Technology , No. 381 Wushan Road, Guangzhou 510640, China.
  • Li XH; School of Chemistry and Chemical Engineering, South China University of Technology , No. 381 Wushan Road, Guangzhou 510640, China.
  • Zong MH; School of Chemistry and Chemical Engineering, South China University of Technology , No. 381 Wushan Road, Guangzhou 510640, China.
  • Lou WY; Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology , No. 381 Wushan Road, Guangzhou 510640, China.
J Agric Food Chem ; 65(10): 2084-2088, 2017 Mar 15.
Article en En | MEDLINE | ID: mdl-28244316
A novel deep eutectic solvent (DES)-DMSO cosolvent system has been, for the first time, successfully used as the reaction medium for the enzymatic acylation of dihydromyricetin (DMY) catalyzed by the immobilized lipase from Aspergillus niger (ANL). The cosolvent mixture, ChCl:Glycerol-DMSO (1:3, v/v) proved to be the optimal medium. With the newly developed cosolvent, the initial reaction rate of enzymatic acylation of DMY achieved 11.1 mM/h and the conversion of DMY was 91.6%. ANL@PD-MNPs is stable and recyclable in this cosolvent, offering 90% conversion rate after repeated use of 5 times. The lipid-solubility of DMY-16-acetate was 10 times higher than that of its raw materials DMY. The results showed that the DMY-16-acetate product exhibits good antioxidative activity. The present research illustrated that the use of DES-DMSO cosolvent may become a feasible alternative for the synthesis of DMY ester.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aspergillus niger / Proteínas Fúngicas / Flavonoles / Lipasa Idioma: En Revista: J Agric Food Chem Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aspergillus niger / Proteínas Fúngicas / Flavonoles / Lipasa Idioma: En Revista: J Agric Food Chem Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos