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Cytoplasmic versus periplasmic expression of site-specifically and bioorthogonally functionalized nanobodies using expressed protein ligation.
Billen, Brecht; Vincke, Cécile; Hansen, Rebekka; Devoogdt, Nick; Muyldermans, Serge; Adriaensens, Peter; Guedens, Wanda.
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  • Billen B; Biomolecule Design Group, Institute for Materials Research (IMO), Hasselt University, BE-3590 Diepenbeek, Belgium. Electronic address: brecht.billen@uhasselt.be.
  • Vincke C; Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel (VUB), BE-1050 Brussels, Belgium. Electronic address: cvincke@vub.ac.be.
  • Hansen R; Biomolecule Design Group, Institute for Materials Research (IMO), Hasselt University, BE-3590 Diepenbeek, Belgium. Electronic address: rebekka.hansen@uhasselt.be.
  • Devoogdt N; In Vivo Cellular and Molecular Imaging Laboratory, Vrije Universiteit Brussel (VUB), BE-1090 Brussels, Belgium. Electronic address: ndevoogd@vub.ac.be.
  • Muyldermans S; Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel (VUB), BE-1050 Brussels, Belgium. Electronic address: svmuylde@vub.ac.be.
  • Adriaensens P; Biomolecule Design Group, Institute for Materials Research (IMO), Hasselt University, BE-3590 Diepenbeek, Belgium; Applied and Analytical Chemistry, Institute for Materials Research (IMO), Hasselt University, BE-3590 Diepenbeek, Belgium. Electronic address: peter.adriaensens@uhasselt.be.
  • Guedens W; Biomolecule Design Group, Institute for Materials Research (IMO), Hasselt University, BE-3590 Diepenbeek, Belgium. Electronic address: wanda.guedens@uhasselt.be.
Protein Expr Purif ; 133: 25-34, 2017 05.
Article en En | MEDLINE | ID: mdl-28238765
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Expresión Génica / Periplasma / Citoplasma / Escherichia coli / Anticuerpos de Dominio Único Idioma: En Revista: Protein Expr Purif Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Expresión Génica / Periplasma / Citoplasma / Escherichia coli / Anticuerpos de Dominio Único Idioma: En Revista: Protein Expr Purif Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos