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Reversible Lifting of Surface Supported Lipid Bilayers with a Membrane-Spanning Nonionic Triblock Copolymer.
Hayden, Steven C; Junghans, Ann; Majewski, Jaroslaw; Firestone, Millicent A.
Afiliación
  • Hayden SC; Materials Physics & Applications, Center for Integrated Nanotechnologies (CINT), Los Alamos National Laboratory , Mail Stop K771, Los Alamos, New Mexico 87545, United States.
  • Junghans A; Lujan Neutron Scattering Center, Los Alamos Neutron Science Center (LANSCE), Los Alamos National Laboratory , Mail Stop H805, Los Alamos, New Mexico 87545, United States.
  • Majewski J; Materials Science & Engineering (MST-7), Los Alamos National Laboratory , Mail Stop H805, Los Alamos, New Mexico 87545, United States.
  • Firestone MA; Materials Physics & Applications, Center for Integrated Nanotechnologies (CINT), Los Alamos National Laboratory , Mail Stop K771, Los Alamos, New Mexico 87545, United States.
Biomacromolecules ; 18(4): 1097-1107, 2017 04 10.
Article en En | MEDLINE | ID: mdl-28225603
Neutron reflectometry was used to monitor structural variations in surface-supported dimyristoylphosphatidycholine (DMPC) bilayers induced by the addition of Triton X-100, a surfactant commonly used to aid solubilization of membrane proteins, and the coaddition of a membrane spanning nonionic amphiphilic triblock copolymer, (PEO117-PPO47-PEO117, Pluronic F98). Surfactant addition causes slight compression of the bilayer thickness and the creation of a distinct EO layer that increases the hydrophilic layer proximal to the supporting substrate (i.e., a water and EO gap between the lipid bilayer and quartz) to 6.8 ± 0.4 Å. Addition of the triblock copolymer into the DMPC:Triton X-100 bilayer increases the complexity of (broadens) the lipid phase transition, further compresses the bilayer, and continues to expand the proximal hydrophilic layer thickness. The observed structural changes are temperature dependent with transmembrane polymer insertion achieved at 37 °C, leading to a compressed membrane thickness of 39.2 ± 0.2 Å and proximal gap of 45.0 ± 0.2 Å. Temperature-driven exclusion of the polymer at 15 °C causes partitioning of the polymer into the proximal space generating a large hydrogel cushion 162 ± 16 Å thick. An intermediate gap width (10-27 Å) is achieved at room temperature (22-25 °C). The temperature-driven changes in the proximal hydrophilic gap dimensions are shown to be reversible, but thermal history causes variation in magnitude. Temperature-driven changes in polymer association with a supported lipid bilayer offer a facile means to reversibly control both the membrane characteristics as well as the separation between membrane and solid substrate.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Membrana Dobles de Lípidos Idioma: En Revista: Biomacromolecules Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Membrana Dobles de Lípidos Idioma: En Revista: Biomacromolecules Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos