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Exposure to cadmium during in vitro maturation at environmental nanomolar levels impairs oocyte fertilization through oxidative damage: A large animal model study.
Martino, N A; Marzano, G; Mangiacotti, M; Miedico, O; Sardanelli, A M; Gnoni, A; Lacalandra, G M; Chiaravalle, A E; Ciani, E; Bogliolo, L; Minervini, F; Pizzi, F; Dell'Aquila, M E.
Afiliación
  • Martino NA; Istituto Zooprofilattico Sperimentale di Puglia e Basilicata, Via Manfredonia 20, 71121 Foggia, Italy; Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari Aldo Moro, Str. Prov. Casamassima Km 3, 70010, Valenzano, Bari, Italy. Electronic address: nicmartino@libero.it.
  • Marzano G; Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari Aldo Moro, Str. Prov. Casamassima Km 3, 70010, Valenzano, Bari, Italy. Electronic address: ros.marzano@gmail.com.
  • Mangiacotti M; Istituto Zooprofilattico Sperimentale di Puglia e Basilicata, Via Manfredonia 20, 71121 Foggia, Italy. Electronic address: michele.mangiacotti@izspb.it.
  • Miedico O; Istituto Zooprofilattico Sperimentale di Puglia e Basilicata, Via Manfredonia 20, 71121 Foggia, Italy. Electronic address: oto.miedico@izspb.it.
  • Sardanelli AM; Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari Aldo Moro, P.zza G. Cesare, 11 70124 Bari, Italy; Institute of Translational Pharmacology (IFT), National Research Council of Italy (CNR), via del Fosso del Cavaliere 100, 00133 Rome, Italy. Electronic address:
  • Gnoni A; Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari Aldo Moro, P.zza G. Cesare, 11 70124 Bari, Italy. Electronic address: antonio.gnoni@uniba.it.
  • Lacalandra GM; Department of Emergency and Organ Transplantation, University of Bari Aldo Moro, Str. Prov. Casamassima Km 3, 70010, Valenzano, Bari, Italy. Electronic address: giovannimichele.lacalandra@uniba.it.
  • Chiaravalle AE; Istituto Zooprofilattico Sperimentale di Puglia e Basilicata, Via Manfredonia 20, 71121 Foggia, Italy. Electronic address: eugenio.chiaravalle@izspb.it.
  • Ciani E; Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari Aldo Moro, Str. Prov. Casamassima Km 3, 70010, Valenzano, Bari, Italy. Electronic address: elena.ciani@uniba.it.
  • Bogliolo L; Obstetrics and Gynaecology Section, Department of Veterinary Medicine, Via Vienna, n°2 07100 Sassari, Italy. Electronic address: luis@uniss.it.
  • Minervini F; Istituto di Scienze delle Produzioni Alimentari (ISPA), National Research Council of Italy (CNR), Via G. Amendola 122/O, 70125 Bari (BA), Italy. Electronic address: fiorenza.minervini@ispa.cnr.it.
  • Pizzi F; Istituto di Biologia e Biotecnologia Agraria (IBBA), National Research Council of Italy (CNR), Sede di Lodi-Via A. Einstein, 26500 Lodi, Italy. Electronic address: pizzi@ibba.cnr.it.
  • Dell'Aquila ME; Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari Aldo Moro, Str. Prov. Casamassima Km 3, 70010, Valenzano, Bari, Italy. Electronic address: mariaelena.dellaquila@uniba.it.
Reprod Toxicol ; 69: 132-145, 2017 04.
Article en En | MEDLINE | ID: mdl-28188904
Cadmium is a highly toxic heavy metal with negative effects on oocyte fertilization. The aim of this study was to analyse whether cadmium-induced impairment of fertilization is caused by mitochondria dysfunction and oxidative stress in the cumulus-oocyte complex (COC). Preliminarily, 19 trace element levels were measured in ovaries from juvenile and adult ewes and age-related cadmium ovarian bioaccumulation at nanomolar concentrations was found. COCs from juvenile and adult ewes, exposed during in vitro maturation to 1nM or 100nM CdCl2, and subjected to in vitro fertilization showed significantly lower fertilization rates in exposed COCs compared with controls. In vitro matured exposed and control COCs underwent confocal microscopy analysis of mitochondria activity and reactive oxygen species (ROS) levels and lipid peroxidation (LPO) assay at cumulus cell and oocyte level. In both age groups, cadmium at nanomolar concentrations induced cumulus-oocyte mitochondria over-activity and oxidative damage which were related to impaired oocyte fertilization.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Cadmio / Fertilización In Vitro Límite: Animals Idioma: En Revista: Reprod Toxicol Asunto de la revista: EMBRIOLOGIA / MEDICINA REPRODUTIVA / TOXICOLOGIA Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Cadmio / Fertilización In Vitro Límite: Animals Idioma: En Revista: Reprod Toxicol Asunto de la revista: EMBRIOLOGIA / MEDICINA REPRODUTIVA / TOXICOLOGIA Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos