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Redox regulation of methionine in calmodulin affects the activity levels of senescence-related transcription factors in litchi.
Jiang, Guoxiang; Xiao, Lu; Yan, Huiling; Zhang, Dandan; Wu, Fuwang; Liu, Xuncheng; Su, Xinguo; Dong, Xinhong; Wang, Jiasheng; Duan, Xuewu; Jiang, Yueming.
Afiliación
  • Jiang G; Guangdong Provincial Key Laboratory of Applied Botany/Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
  • Xiao L; Guangdong Provincial Key Laboratory of Applied Botany/Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Yan H; Guangdong Provincial Key Laboratory of Applied Botany/Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Zhang D; Guangdong Provincial Key Laboratory of Applied Botany/Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
  • Wu F; Guangdong Provincial Key Laboratory of Applied Botany/Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
  • Liu X; Guangdong Provincial Key Laboratory of Applied Botany/Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
  • Su X; Guangdong Food and Drug Vocational College, Guangzhou 510520, China.
  • Dong X; College of Chemistry and Biology Engineering, Guilin University of Technology, Gulin 541004, China.
  • Wang J; Department of Environmental Health Science, College of Public Health, University of Georgia, Athens, GA 30602, USA.
  • Duan X; Guangdong Provincial Key Laboratory of Applied Botany/Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China. Electronic address: xwduan@scbg.ac.cn.
  • Jiang Y; Guangdong Provincial Key Laboratory of Applied Botany/Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
Biochim Biophys Acta Gen Subj ; 1861(5 Pt A): 1140-1151, 2017 May.
Article en En | MEDLINE | ID: mdl-28188859
Reactive oxygen species (ROS) play a role in aging and senescence in organisms. The oxidation of methionine (Met) residues in proteins to Met sulfoxide by ROS can cause conformational alteration and functional impairments. Met oxidation is reversed by Met sulfoxide reductase (Msr) A and B. Currently, the repair of oxidized proteins by Msr and Msr-mediated physiological functions are not well understood, especially in higher plants. The down-regulated expression of LcMsrA1/B1 may be involved in the senescence of litchi (Litchi chinensis) fruit. We verified that LcCaM1 is a substrate of LcMsrA1 and LcMsrB1 in vitro and in vivo, and oxidized LcCaM1 could be repaired by LcMsrA1 in combination with LcMsrB1. Moreover, LcMsrA1 and LcMsrB1 play important roles in repairing oxidized Met110 and Met125 residues, respectively, in LcCaM1. Furthermore, the Met oxidation in LcCaM1 did not affect its physical interactions with two LcCaM1-binding senescence-related transcription factors LcNAC13 and LcWRKY1, but enhanced their DNA-binding activities. Therefore, we hypothesized that the down-regulated expression of LcMsrA1/B1 results in the accelerated oxidation of LcCaM1, which enhanced the DNA-binding activities of LcNAC13 and LcWRKY1, thereby activating or repressing the expression of senescence-related genes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Calmodulina / Senescencia Celular / Litchi / Metionina Idioma: En Revista: Biochim Biophys Acta Gen Subj Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Calmodulina / Senescencia Celular / Litchi / Metionina Idioma: En Revista: Biochim Biophys Acta Gen Subj Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos