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Rapid Chair-Side Test for Detection of Porphyromonas gingivalis.
O'Brien-Simpson, N M; Burgess, K; Lenzo, J C; Brammar, G C; Darby, I B; Reynolds, E C.
Afiliación
  • O'Brien-Simpson NM; 1 Oral Health Cooperative Research Centre, Melbourne Dental School, Bio21 Institute, The University of Melbourne, Victoria, Australia.
  • Burgess K; 1 Oral Health Cooperative Research Centre, Melbourne Dental School, Bio21 Institute, The University of Melbourne, Victoria, Australia.
  • Lenzo JC; 1 Oral Health Cooperative Research Centre, Melbourne Dental School, Bio21 Institute, The University of Melbourne, Victoria, Australia.
  • Brammar GC; 1 Oral Health Cooperative Research Centre, Melbourne Dental School, Bio21 Institute, The University of Melbourne, Victoria, Australia.
  • Darby IB; 1 Oral Health Cooperative Research Centre, Melbourne Dental School, Bio21 Institute, The University of Melbourne, Victoria, Australia.
  • Reynolds EC; 1 Oral Health Cooperative Research Centre, Melbourne Dental School, Bio21 Institute, The University of Melbourne, Victoria, Australia.
J Dent Res ; 96(6): 618-625, 2017 Jun.
Article en En | MEDLINE | ID: mdl-28182517
Porphyromonas gingivalis is a keystone pathogen of chronic periodontitis, and its intraoral levels have been shown to predict disease progression (activity). An accurate and sensitive chair-side (point of care) test to determine disease activity is critical for early intervention and clinical management of disease. This study aimed to develop a rapid, chair-side, saliva-based detection of P. gingivalis. Monoclonal antibodies (mAbs) to the A1-adhesin domain of the P. gingivalis RgpA-Kgp proteinase-adhesin complex were screened by enzyme-linked immunosorbent assay and microbial flow cytometry, with 2 mAbs shown to recognize all laboratory and clinical strains tested, without significantly cross-reacting with other oral bacteria tested. With these mAbs, an immunochromatographic device was produced and shown in preclinical studies to detect, in inoculated saliva, all P. gingivalis laboratory strains and clinical isolates tested. The device was able to detect ≥1 × 105 P. gingivalis cells/mL. In a patient age- and sex-matched control clinical cohort, P. gingivalis levels in saliva-as measured by real-time polymerase chain reaction-positively correlated with P. gingivalis levels in subgingival plaque ( r = 0.819, P < 0.01) and clinical parameters of disease ( r = 0.633, P < 0.01). A positive device result strongly correlated with P. gingivalis levels >1 × 105 cells/mL in saliva ( r = 0.778, P < 0.001) and subgingival plaque ( r = 0.715, P < 0.001) with sensitivity, specificity, positive/negative predictive values, and accuracy levels of 95.0%, 93.3%, 90.5%, 96.6%, and 94.0%, respectively. The device result also positively correlated ( r = 0.695, P < 0.01) with disease severity as measured by probing depth. Detection of P. gingivalis in saliva was found to be rapid, taking 3 min from sample collection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saliva / Técnicas Bacteriológicas / Porphyromonas gingivalis / Sistemas de Atención de Punto / Placa Dental / Periodontitis Crónica Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: J Dent Res Año: 2017 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saliva / Técnicas Bacteriológicas / Porphyromonas gingivalis / Sistemas de Atención de Punto / Placa Dental / Periodontitis Crónica Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: J Dent Res Año: 2017 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos