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Molecular characterization and functional analyses of a diapause hormone receptor-like gene in parthenogenetic Artemia.
Ye, Hui-Li; Li, Dong-Rui; Yang, Jin-Shu; Chen, Dian-Fu; De Vos, Stephanie; Vuylsteke, Marnik; Sorgeloos, Patrick; Van Stappen, Gilbert; Bossier, Peter; Nagasawa, Hiromichi; Yang, Wei-Jun.
Afiliación
  • Ye HL; College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, PR China.
  • Li DR; College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, PR China.
  • Yang JS; College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, PR China.
  • Chen DF; College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, PR China.
  • De Vos S; Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium.
  • Vuylsteke M; Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium.
  • Sorgeloos P; Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium.
  • Van Stappen G; Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium.
  • Bossier P; Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium.
  • Nagasawa H; Department of Applied Biological Chemistry, The University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8657, Japan. Electronic address: anagahi@mail.ecc.u-tokyo.ac.jp.
  • Yang WJ; College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, PR China. Electronic address: w_jyang@zju.edu.cn.
Peptides ; 90: 100-110, 2017 04.
Article en En | MEDLINE | ID: mdl-28174072
In arthropods, mature females under certain conditions produce and release encysted gastrula embryos that enter diapause, a state of obligate dormancy. The process is presumably regulated by diapause hormone (DH) and diapause hormone receptor (DHR) that were identified in the silkworm, Bombyx mori and other insects. However, the molecular structure and function of DHR in crustaceans remains unknown. Here, a DHR-like gene from parthenogenetic Artemia (Ar-DHR) was isolated and sequenced. The cDNA sequence consists of 1410bp with a 1260-bp open reading frame encoding a protein consisting of 420 amino acid residues. The results of real-time PCR (qRT-PCR) and Western blot analysis showed that the mRNA and protein of Ar-DHR were mainly expressed at the diapause stage. Furthermore, we found that Ar-DHR was located on the cell membrane of the pre-diapause cyst but in the cytoplasm of the diapause cyst by analysis of immunofluorescence. In vivo knockdown of Ar-DHR by RNA interference (RNAi) and antiserum neutralization consistently inhibited diapause cysts formation. The results indicated that Ar-DHR plays an important role in the induction and maintenance of embryonic diapause in Artemia. Thus, our findings provide an insight into the regulation of diapause formation in Artemia and the function of Ar-DHR.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Artemia / Neuropéptidos / Diapausa de Insecto Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Peptides Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Artemia / Neuropéptidos / Diapausa de Insecto Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Peptides Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos