Your browser doesn't support javascript.
loading
MicroRNA-211 and autophagy-related gene 14 signaling regulate osteoblast-like cell differentiation of human induced pluripotent stem cells.
Ozeki, Nobuaki; Hase, Naoko; Hiyama, Taiki; Yamaguchi, Hideyuki; Kawai-Asano, Rie; Nakata, Kazuhiko; Mogi, Makio.
Afiliación
  • Ozeki N; Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
  • Hase N; Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
  • Hiyama T; Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
  • Yamaguchi H; Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
  • Kawai-Asano R; Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
  • Nakata K; Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
  • Mogi M; Department of Integrative Education of Pharmacy, School of Pharmacy, Aichi Gakuin University, Nagoya, Aichi 464-8650, Japan. Electronic address: makio@dpc.agu.ac.jp.
Exp Cell Res ; 352(1): 63-74, 2017 03 01.
Article en En | MEDLINE | ID: mdl-28159471
MicroRNAs (miRNAs) have been the subject of recent attention as key regulatory factors in cell differentiation. In the current study, to explore the early signaling cascade of osteogenic differentiation of human induced pluripotent stem (hiPS) cells, we investigated miR-211 regulation and autophagy-related gene (Atg) signaling in osteogenic differentiation. In addition to reciprocal strong induction of miR-211 expression in differentiated cells following osteogenic differentiation, we found abundant Argonaute 3 bound to miR-211. There were also dramatic increases in the mRNA and protein levels of Atg14 together with increases in the amount of autophagosomes as well as autophagic fluxes. While transfection of a miR-211 inhibitor abrogated the induction of Atg14, autophagy events, osteoblast differentiation markers, and induction of calcification were suppressed markedly. Treatment with small interfering RNAs against Atg14 also suppressed the osteogenic differentiation medium (ODM)-induced increase in osteogenic differentiation. The osteogenic phenotype was inhibited by chloroquine (an autophagy inhibitor), but increased after treatment with rapamycin (an autophagy inducer). Taken together with our previous findings, we have revealed a unique sequential cascade involving miR-211 and Atg14 in ODM-induced differentiation of hiPS cells into osteoblast-like cells at a relatively early stage.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoblastos / Autofagia / Diferenciación Celular / Proteínas Adaptadoras del Transporte Vesicular / MicroARNs / Células Madre Pluripotentes Inducidas / Proteínas Relacionadas con la Autofagia Límite: Humans Idioma: En Revista: Exp Cell Res Año: 2017 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoblastos / Autofagia / Diferenciación Celular / Proteínas Adaptadoras del Transporte Vesicular / MicroARNs / Células Madre Pluripotentes Inducidas / Proteínas Relacionadas con la Autofagia Límite: Humans Idioma: En Revista: Exp Cell Res Año: 2017 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos