Induction of cytochrome P-450 isozymes by chromanamine derivatives in rat liver.
Xenobiotica
; 19(8): 901-12, 1989 Aug.
Article
en En
| MEDLINE
| ID: mdl-2815832
1. Pretreatment of rats with 6-(3-picolyl)amino-2,2,5,8-tetramethylchromane (PATC) for 7 days resulted in a significant increase in the activities of benzphetamine N-demethylase, p-nitroanisole O-demethylase and aniline hydroxylase in liver microsomes prepared 24 h after the last treatment. 2. Analysis by Western blot showed that PATC induces cytochrome P-450 b, P-450 c and P-450 d, which are the major forms of cytochrome P-450 in liver microsomes of rats when pretreated with phenobarbital and 3-methylcholanthrene. 3. Exposure of liver sections to the antibodies to cytochrome P-450 b and P-450 c resulted in intense immunostaining within the centrilobular regions, but produced staining of considerably weaker intensity in the perilobular region. Semiquantitative immunochemical analysis, by image analyser, of cytochrome P-450 b and P-450 c showed that centrilobular hepatocytes were stained more intensively than perilobular hepatocytes. 4. These results indicate that PATC induces cytochromes P-450 b and P-450 c, in the centrilobular hepatocytes to a greater degree than those in the perilobular hepatocytes. 5. Co-administration of PATC with pentobarbital caused a significant increase in pentobarbital sleeping time. Furthermore, PATC was found to cause a decrease in the activity of benzphetamine N-demethylase in liver microsomes prepared 30 min after treatment with the drug.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Picolinas
/
Benzopiranos
/
Cromanos
/
Sistema Enzimático del Citocromo P-450
/
Hígado
Límite:
Animals
Idioma:
En
Revista:
Xenobiotica
Año:
1989
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Reino Unido