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Imaging and structural studies of DNA-protein complexes and membrane ion channels.
Marini, M; Limongi, T; Falqui, A; Genovese, A; Allione, M; Moretti, M; Lopatin, S; Tirinato, L; Das, G; Torre, B; Giugni, A; Cesca, F; Benfenati, F; Di Fabrizio, E.
Afiliación
  • Marini M; King Abdullah University of Science and Technology, SMILEs lab, PSE and BESE Divisions, Thuwal, 23955-6900, Kingdom of Saudi Arabia. enzo.difabrizio@kaust.edu.sa.
  • Limongi T; King Abdullah University of Science and Technology, SMILEs lab, PSE and BESE Divisions, Thuwal, 23955-6900, Kingdom of Saudi Arabia. enzo.difabrizio@kaust.edu.sa.
  • Falqui A; King Abdullah University of Science and Technology, NABLA lab, BESE Division, Thuwal, 23955-6900, Kingdom of Saudi Arabia.
  • Genovese A; King Abdullah University of Science and Technology, NABLA lab, BESE Division, Thuwal, 23955-6900, Kingdom of Saudi Arabia.
  • Allione M; King Abdullah University of Science and Technology, SMILEs lab, PSE and BESE Divisions, Thuwal, 23955-6900, Kingdom of Saudi Arabia. enzo.difabrizio@kaust.edu.sa.
  • Moretti M; King Abdullah University of Science and Technology, SMILEs lab, PSE and BESE Divisions, Thuwal, 23955-6900, Kingdom of Saudi Arabia. enzo.difabrizio@kaust.edu.sa.
  • Lopatin S; King Abdullah University of Science and Technology, Imaging and Characterization core lab, Thuwal, 23955-6900, Kingdom of Saudi Arabia.
  • Tirinato L; King Abdullah University of Science and Technology, SMILEs lab, PSE and BESE Divisions, Thuwal, 23955-6900, Kingdom of Saudi Arabia. enzo.difabrizio@kaust.edu.sa.
  • Das G; King Abdullah University of Science and Technology, SMILEs lab, PSE and BESE Divisions, Thuwal, 23955-6900, Kingdom of Saudi Arabia. enzo.difabrizio@kaust.edu.sa.
  • Torre B; King Abdullah University of Science and Technology, SMILEs lab, PSE and BESE Divisions, Thuwal, 23955-6900, Kingdom of Saudi Arabia. enzo.difabrizio@kaust.edu.sa.
  • Giugni A; King Abdullah University of Science and Technology, SMILEs lab, PSE and BESE Divisions, Thuwal, 23955-6900, Kingdom of Saudi Arabia. enzo.difabrizio@kaust.edu.sa.
  • Cesca F; Center for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia, Genova, Italy.
  • Benfenati F; Center for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia, Genova, Italy.
  • Di Fabrizio E; King Abdullah University of Science and Technology, SMILEs lab, PSE and BESE Divisions, Thuwal, 23955-6900, Kingdom of Saudi Arabia. enzo.difabrizio@kaust.edu.sa.
Nanoscale ; 9(8): 2768-2777, 2017 Feb 23.
Article en En | MEDLINE | ID: mdl-28155926
In bio-imaging by electron microscopy, damage of the sample and limited contrast are the two main hurdles for reaching high image quality. We extend a new preparation method based on nanofabrication and super-hydrophobicity to the imaging and structural studies of nucleic acids, nucleic acid-protein complexes (DNA/Rad51 repair protein complex) and neuronal ion channels (gap-junction, K+ and GABAA channels) as paradigms of biological significance and increasing complexity. The preparation method is based on the liquid phase and is compatible with physiological conditions. Only in the very last stage, samples are dried for TEM analysis. Conventional TEM and high-resolution TEM (HRTEM) were used to achieve a resolution of 3.3 and 1.5 Å, respectively. The EM dataset quality allows the determination of relevant structural and metrological information on the DNA structure, DNA-protein interactions and ion channels, allowing the identification of specific macromolecules and their structure.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Ácidos Nucleicos / Microscopía Electrónica de Transmisión / Canales Iónicos Límite: Animals Idioma: En Revista: Nanoscale Año: 2017 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Ácidos Nucleicos / Microscopía Electrónica de Transmisión / Canales Iónicos Límite: Animals Idioma: En Revista: Nanoscale Año: 2017 Tipo del documento: Article Pais de publicación: Reino Unido