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JMJD-1.2/PHF8 controls axon guidance by regulating Hedgehog-like signaling.
Riveiro, Alba Redo; Mariani, Luca; Malmberg, Emily; Amendola, Pier Giorgio; Peltonen, Juhani; Wong, Garry; Salcini, Anna Elisabetta.
Afiliación
  • Riveiro AR; Biotech Research & Innovation Centre (BRIC), University of Copenhagen, 2200, Copenhagen, Denmark.
  • Mariani L; Centre for Epigenetics, University of Copenhagen, 2200, Copenhagen, Denmark.
  • Malmberg E; Biotech Research & Innovation Centre (BRIC), University of Copenhagen, 2200, Copenhagen, Denmark.
  • Amendola PG; Centre for Epigenetics, University of Copenhagen, 2200, Copenhagen, Denmark.
  • Peltonen J; Biotech Research & Innovation Centre (BRIC), University of Copenhagen, 2200, Copenhagen, Denmark.
  • Wong G; Centre for Epigenetics, University of Copenhagen, 2200, Copenhagen, Denmark.
  • Salcini AE; Biotech Research & Innovation Centre (BRIC), University of Copenhagen, 2200, Copenhagen, Denmark.
Development ; 144(5): 856-865, 2017 03 01.
Article en En | MEDLINE | ID: mdl-28126843
Components of the KDM7 family of histone demethylases are implicated in neuronal development and one member, PHF8, is often found to be mutated in cases of X-linked mental retardation. However, how PHF8 regulates neurodevelopmental processes and contributes to the disease is still largely unknown. Here, we show that the catalytic activity of a PHF8 homolog in Caenorhabditis elegans, JMJD-1.2, is required non-cell-autonomously for proper axon guidance. Loss of JMJD-1.2 dysregulates transcription of the Hedgehog-related genes wrt-8 and grl-16, the overexpression of which is sufficient to induce the axonal defects. Deficiency of either wrt-8 or grl-16, or reduced expression of homologs of genes promoting Hedgehog signaling, restores correct axon guidance in jmjd-1.2 mutants. Genetic and overexpression data indicate that Hedgehog-related genes act on axon guidance through actin remodelers. Thus, our study highlights a novel function of jmjd-1.2 in axon guidance that might be relevant for the onset of X-linked mental retardation and provides compelling evidence of a conserved function of the Hedgehog pathway in C. elegans axon migration.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Axones / Caenorhabditis elegans / Regulación del Desarrollo de la Expresión Génica / Proteínas de Caenorhabditis elegans / Proteínas Hedgehog / Histona Demetilasas con Dominio de Jumonji / Orientación del Axón Tipo de estudio: Guideline Límite: Animals Idioma: En Revista: Development Asunto de la revista: BIOLOGIA / EMBRIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Axones / Caenorhabditis elegans / Regulación del Desarrollo de la Expresión Génica / Proteínas de Caenorhabditis elegans / Proteínas Hedgehog / Histona Demetilasas con Dominio de Jumonji / Orientación del Axón Tipo de estudio: Guideline Límite: Animals Idioma: En Revista: Development Asunto de la revista: BIOLOGIA / EMBRIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Reino Unido