Your browser doesn't support javascript.
loading
Integrative miRNA and mRNA analysis in penile carcinomas reveals markers and pathways with potential clinical impact.
Kuasne, Hellen; Barros-Filho, Mateus C; Busso-Lopes, Ariane; Marchi, Fábio A; Pinheiro, Maisa; Muñoz, Juan J M; Scapulatempo-Neto, Cristovam; Faria, Eliney F; Guimarães, Gustavo C; Lopes, Ademar; Trindade-Filho, José C S; Domingues, Maria Aparecida C; Drigo, Sandra A; Rogatto, Silvia R.
Afiliación
  • Kuasne H; CIPE-A. C. Camargo Cancer Center, São Paulo, Brazil.
  • Barros-Filho MC; Department of Urology, Faculty of Medicine, São Paulo State University-UNESP, Botucatu, São Paulo, Brazil.
  • Busso-Lopes A; CIPE-A. C. Camargo Cancer Center, São Paulo, Brazil.
  • Marchi FA; CIPE-A. C. Camargo Cancer Center, São Paulo, Brazil.
  • Pinheiro M; CIPE-A. C. Camargo Cancer Center, São Paulo, Brazil.
  • Muñoz JJ; CIPE-A. C. Camargo Cancer Center, São Paulo, Brazil.
  • Scapulatempo-Neto C; CIPE-A. C. Camargo Cancer Center, São Paulo, Brazil.
  • Faria EF; Department of Pathology, Americas Centro Oncológico Integrado, Rio de Janeiro, Brazil.
  • Guimarães GC; Department of Urology, Barretos Cancer Hospital, Barretos, São Paulo, Brazil.
  • Lopes A; Department of Urology, A. C. Camargo Cancer Center, São Paulo, Brazil.
  • Trindade-Filho JC; Department of Urology, A. C. Camargo Cancer Center, São Paulo, Brazil.
  • Domingues MA; Department of Urology, Faculty of Medicine, São Paulo State University-UNESP, Botucatu, São Paulo, Brazil.
  • Drigo SA; Department of Pathology, Faculty of Medicine, São Paulo State University-UNESP, Botucatu, São Paulo, Brazil.
  • Rogatto SR; Department of Urology, Faculty of Medicine, São Paulo State University-UNESP, Botucatu, São Paulo, Brazil.
Oncotarget ; 8(9): 15294-15306, 2017 Feb 28.
Article en En | MEDLINE | ID: mdl-28122331
Penile carcinoma (PeCa) is an important public health issue in poor and developing countries, and has only recently been explored in terms of genetic and epigenetic studies. Integrative data analysis is a powerful method for the identification of molecular drivers involved in cancer development and progression. miRNA and mRNA expression profiles followed by integrative analysis were investigated in 23 PeCa and 12 non-neoplastic penile tissues (NPT). Expression levels of eight miRNAs and 10 mRNAs were evaluated in the same set of samples used for microarray and in a validation set of cases (PeCa = 36; NPT = 27). Eighty-one miRNAs and 2,697 mRNAs were identified as differentially expressed in PeCa. Integrative data analysis revealed 255 mRNAs potentially regulated by 68 miRNAs. Using RT-qPCR, eight miRNAs and nine transcripts were confirmed as altered in PeCa. We identified that MMP1, MMP12 and PPARG and hsa-miR-31-5p, hsa-miR-224-5p, and hsa-miR-223-3p were able to distinguish tumors from NPT with high sensitivity and specificity. Higher MMP1 expression was detected as a better predictor of lymph node metastasis than the clinical-pathological data. In addition, PPARG and EGFR were highlighted as potential pathways for targeted therapy in PeCa. The analysis based on HPV positivity (7 of 23 cases) revealed five miRNA and 13 mRNA differentially expressed. Although in a limited number of cases, HPV positive PeCa presented less aggressive phenotype in comparison with negative cases. Overall, an integrative analysis using mRNA and miRNA profiles revealed markers related with tumor development and progression. Furthermore, MMP1 expression level was a predictive marker for lymph node metastasis in patients with PeCa.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias del Pene / ARN Mensajero / Transducción de Señal / Biomarcadores de Tumor / Regulación Neoplásica de la Expresión Génica / MicroARNs Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Adult / Aged / Aged80 / Humans / Male / Middle aged Idioma: En Revista: Oncotarget Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias del Pene / ARN Mensajero / Transducción de Señal / Biomarcadores de Tumor / Regulación Neoplásica de la Expresión Génica / MicroARNs Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Adult / Aged / Aged80 / Humans / Male / Middle aged Idioma: En Revista: Oncotarget Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos