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Programmed cell death-1 expression correlates with disease severity and IL-5 in chronic rhinosinusitis with nasal polyps.
Kortekaas Krohn, I; Bobic, S; Dooley, J; Lan, F; Zhang, N; Bachert, C; Steelant, B; Bullens, D M; Liston, A; Ceuppens, J L; Seys, S F; Hellings, P W.
Afiliación
  • Kortekaas Krohn I; Laboratory of Clinical Immunology, Department of Microbiology & Immunology, University of Leuven, Leuven, Belgium.
  • Bobic S; Laboratory of Clinical Immunology, Department of Microbiology & Immunology, University of Leuven, Leuven, Belgium.
  • Dooley J; Translational Immunology Laboratory, VIB, University of Leuven, Leuven, Belgium.
  • Lan F; Upper Airway Research Laboratory, Department of Otorhinolaryngology, University Hospital Ghent, Ghent, Belgium.
  • Zhang N; Upper Airway Research Laboratory, Department of Otorhinolaryngology, University Hospital Ghent, Ghent, Belgium.
  • Bachert C; Upper Airway Research Laboratory, Department of Otorhinolaryngology, University Hospital Ghent, Ghent, Belgium.
  • Steelant B; Laboratory of Clinical Immunology, Department of Microbiology & Immunology, University of Leuven, Leuven, Belgium.
  • Bullens DM; Pediatric Immunology, Department of Microbiology and Immunology, University of Leuven, Leuven, Belgium.
  • Liston A; Clinical Department of Pediatrics, University Hospitals Leuven, Leuven, Belgium.
  • Ceuppens JL; Translational Immunology Laboratory, VIB, University of Leuven, Leuven, Belgium.
  • Seys SF; Laboratory of Clinical Immunology, Department of Microbiology & Immunology, University of Leuven, Leuven, Belgium.
  • Hellings PW; Laboratory of Clinical Immunology, Department of Microbiology & Immunology, University of Leuven, Leuven, Belgium.
Allergy ; 72(6): 985-993, 2017 Jun.
Article en En | MEDLINE | ID: mdl-28122135
BACKGROUND: Programmed cell death-1 (PD-1) is a negative regulator of T-cell responses. Expression of PD-1 and its ligands PD-L1 and PD-L2 in chronic rhinosinusitis with nasal polyps (CRSwNP) is poorly studied. METHODS: Expression of PD-1, PD-L1, PD-L2, TGF-ß, IL-5, and IL-10 mRNA was measured by real-time quantitative PCR on tissue homogenates of patients with CRSwNP (n = 21) and healthy controls (n = 21) and on primary epithelial cells. Disease severity was scored using the Lund-Mackay scores of maxillofacial computed tomography (CT) scans. Expression of PD-1 and PD-L1/L2 was evaluated at the cellular and tissue levels (n = 6) by flow cytometry and immunohistochemistry. RESULTS: Programmed cell death-1 mRNA expression was increased in tissue homogenates from patients with CRSwNP compared with controls, irrespective of the atopy status. Importantly, expression of PD-1 correlated with the total CT scan scores (r = 0.5, P = 0.02). Additionally, a significant association was found between PD-1 mRNA and expression of IL-5 mRNA in control nasal tissue (r = 0.95, P < 0.0001) and in CRSwNP (r = 0.63, P = 0.002). PD-1 was expressed on different subsets of T cells and CD11b- dendritic cells. Both PD-1 and its ligands were expressed on primary epithelial cells from control nasal tissue and nasal polyp tissue. CONCLUSIONS: Higher PD-1 expression was found in CRSwNP than in nasal tissue from controls. This was associated with disease severity and tissue IL-5 expression but unrelated to the patients' atopy status.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sinusitis / Pólipos Nasales / Interleucina-5 / Receptor de Muerte Celular Programada 1 Tipo de estudio: Observational_studies / Risk_factors_studies Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Allergy Año: 2017 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Dinamarca
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sinusitis / Pólipos Nasales / Interleucina-5 / Receptor de Muerte Celular Programada 1 Tipo de estudio: Observational_studies / Risk_factors_studies Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Allergy Año: 2017 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Dinamarca