Engineering 7ß-Hydroxysteroid Dehydrogenase for Enhanced Ursodeoxycholic Acid Production by Multiobjective Directed Evolution.

Zheng, Ming-Min; Chen, Ke-Cai; Wang, Ru-Feng; Li, Hao; Li, Chun-Xiu; Xu, Jian-He

Zheng, Ming-Min; Chen, Ke-Cai; Wang, Ru-Feng; Li, Hao; Li, Chun-Xiu; Xu, Jian-He.

Afiliación

Zheng MM; State Key Laboratory of Bioreactor Engineering and Shanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology , Shanghai 200237, P. R. China.
Chen KC; State Key Laboratory of Bioreactor Engineering and Shanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology , Shanghai 200237, P. R. China.
Wang RF; State Key Laboratory of Bioreactor Engineering and Shanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology , Shanghai 200237, P. R. China.
Li H; State Key Laboratory of Bioreactor Engineering and Shanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology , Shanghai 200237, P. R. China.
Li CX; State Key Laboratory of Bioreactor Engineering and Shanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology , Shanghai 200237, P. R. China.
Xu JH; State Key Laboratory of Bioreactor Engineering and Shanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology , Shanghai 200237, P. R. China.

J Agric Food Chem ; 65(6): 1178-1185, 2017 Feb 15.

Article en En | MEDLINE | ID: mdl-28116898

Asunto(s)

Hidroxiesteroide Deshidrogenasas/genética; Hidroxiesteroide Deshidrogenasas/metabolismo; Ingeniería de Proteínas/métodos; Ácido Ursodesoxicólico/metabolismo; Evolución Molecular Dirigida/métodos; Concentración de Iones de Hidrógeno; Hidroxiesteroide Deshidrogenasas/química; Ácido Litocólico/análogos & derivados; Ácido Litocólico/metabolismo; Mutagénesis Sitio-Dirigida; Reacción en Cadena de la Polimerasa/métodos; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo; Ácido Ursodesoxicólico/biosíntesis

Palabras clave

7ß-hydroxysteroid dehydrogenase; biocatalysis; cascade reaction; multiobjective directed evolution; ursodeoxycholic acid

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácido Ursodesoxicólico / Ingeniería de Proteínas / Hidroxiesteroide Deshidrogenasas Idioma: En Revista: J Agric Food Chem Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos

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