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Differential PI3Kδ Signaling in CD4+ T-cell Subsets Enables Selective Targeting of T Regulatory Cells to Enhance Cancer Immunotherapy.
Ahmad, Shamim; Abu-Eid, Rasha; Shrimali, Rajeev; Webb, Mason; Verma, Vivek; Doroodchi, Atbin; Berrong, Zuzana; Samara, Raed; Rodriguez, Paulo C; Mkrtichyan, Mikayel; Khleif, Samir N.
Afiliación
  • Ahmad S; Georgia Cancer Center, Augusta University, Augusta, Georgia.
  • Abu-Eid R; Georgia Cancer Center, Augusta University, Augusta, Georgia.
  • Shrimali R; University of Aberdeen Dental School, Foresterhill, Aberdeen, Scotland, United Kingdom.
  • Webb M; Georgia Cancer Center, Augusta University, Augusta, Georgia.
  • Verma V; Peloton Therapeutics, Dallas, Texas.
  • Doroodchi A; Georgia Cancer Center, Augusta University, Augusta, Georgia.
  • Berrong Z; Georgia Cancer Center, Augusta University, Augusta, Georgia.
  • Samara R; Georgia Cancer Center, Augusta University, Augusta, Georgia.
  • Rodriguez PC; Georgia Cancer Center, Augusta University, Augusta, Georgia.
  • Mkrtichyan M; R&D Project Management Department, Qiagen, Frederick, Maryland.
  • Khleif SN; Georgia Cancer Center, Augusta University, Augusta, Georgia.
Cancer Res ; 77(8): 1892-1904, 2017 04 15.
Article en En | MEDLINE | ID: mdl-28108509
To modulate T-cell function for cancer therapy, one challenge is to selectively attenuate regulatory but not conventional CD4+ T-cell subsets [regulatory T cell (Treg) and conventional T cell (Tconv)]. In this study, we show how a functional dichotomy in Class IA PI3K isoforms in these two subsets of CD4+ T cells can be exploited to target Treg while leaving Tconv intact. Studies employing isoform-specific PI3K inhibitors and a PI3Kδ-deficient mouse strain revealed that PI3Kα and PI3Kß were functionally redundant with PI3Kδ in Tconv. Conversely, PI3Kδ was functionally critical in Treg, acting there to control T-cell receptor signaling, cell proliferation, and survival. Notably, in a murine model of lung cancer, coadministration of a PI3Kδ-specific inhibitor with a tumor-specific vaccine decreased numbers of suppressive Treg and increased numbers of vaccine-induced CD8 T cells within the tumor microenvironment, eliciting potent antitumor efficacy. Overall, our results offer a mechanistic rationale to employ PI3Kδ inhibitors to selectively target Treg and improve cancer immunotherapy. Cancer Res; 77(8); 1892-904. ©2017 AACR.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T CD4-Positivos / Linfocitos T Reguladores / Fosfatidilinositol 3-Quinasas / Neoplasias Experimentales Límite: Animals Idioma: En Revista: Cancer Res Año: 2017 Tipo del documento: Article País de afiliación: Georgia Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T CD4-Positivos / Linfocitos T Reguladores / Fosfatidilinositol 3-Quinasas / Neoplasias Experimentales Límite: Animals Idioma: En Revista: Cancer Res Año: 2017 Tipo del documento: Article País de afiliación: Georgia Pais de publicación: Estados Unidos