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Comparative Phosphoproteomics Reveals an Important Role of MKK2 in Banana (Musa spp.) Cold Signal Network.
Gao, Jie; Zhang, Sheng; He, Wei-Di; Shao, Xiu-Hong; Li, Chun-Yu; Wei, Yue-Rong; Deng, Gui-Ming; Kuang, Rui-Bin; Hu, Chun-Hua; Yi, Gan-Jun; Yang, Qiao-Song.
Afiliación
  • Gao J; State Key Laboratory for Conservation and Utilization of Subtropical Agro- bioresources, South China Agricultural University, Guangzhou, 510640, China.
  • Zhang S; Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
  • He WD; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, 510640, China.
  • Shao XH; The Guangzhou Research Branch of the National Banana Improvement Center, Guangzhou, 510640, China.
  • Li CY; Institute of Biotechnology, Cornell University, Ithaca, NY, USA.
  • Wei YR; Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
  • Deng GM; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, 510640, China.
  • Kuang RB; The Guangzhou Research Branch of the National Banana Improvement Center, Guangzhou, 510640, China.
  • Hu CH; Key Laboratory of Horticultural Plant Biology of the Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China.
  • Yi GJ; Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
  • Yang QS; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, 510640, China.
Sci Rep ; 7: 40852, 2017 01 20.
Article en En | MEDLINE | ID: mdl-28106078
Low temperature is one of the key environmental stresses, which greatly affects global banana production. However, little is known about the global phosphoproteomes in Musa spp. and their regulatory roles in response to cold stress. In this study, we conducted a comparative phosphoproteomic profiling of cold-sensitive Cavendish Banana and relatively cold tolerant Dajiao under cold stress. Phosphopeptide abundances of five phosphoproteins involved in MKK2 interaction network, including MKK2, HY5, CaSR, STN7 and kinesin-like protein, show a remarkable difference between Cavendish Banana and Dajiao in response to cold stress. Western blotting of MKK2 protein and its T31 phosphorylated peptide verified the phosphoproteomic results of increased T31 phosphopeptide abundance with decreased MKK2 abundance in Daojiao for a time course of cold stress. Meanwhile increased expression of MKK2 with no detectable T31 phosphorylation was found in Cavendish Banana. These results suggest that the MKK2 pathway in Dajiao, along with other cold-specific phosphoproteins, appears to be associated with the molecular mechanisms of high tolerance to cold stress in Dajiao. The results also provide new evidence that the signaling pathway of cellular MKK2 phosphorylation plays an important role in abiotic stress tolerance that likely serves as a universal plant cold tolerance mechanism.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfoproteínas / Proteínas de Plantas / Frío / Quinasas de Proteína Quinasa Activadas por Mitógenos / Proteoma / Musa / Proteómica Idioma: En Revista: Sci Rep Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfoproteínas / Proteínas de Plantas / Frío / Quinasas de Proteína Quinasa Activadas por Mitógenos / Proteoma / Musa / Proteómica Idioma: En Revista: Sci Rep Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido