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Non-polar lipids accumulate during storage of transfusion products and do not contribute to the onset of transfusion-related acute lung injury.
Peters, A L; Vervaart, M A T; van Bruggen, R; de Korte, D; Nieuwland, R; Kulik, W; Vlaar, A P J.
Afiliación
  • Peters AL; Laboratory of Experimental Intensive Care and Anesthesia, Academic Medical Center, Amsterdam, The Netherlands.
  • Vervaart MA; Department of Intensive Care, Academic Medical Center, Amsterdam, The Netherlands.
  • van Bruggen R; Laboratory Genetic Metabolic Diseases, Academic Medical Center, Amsterdam, The Netherlands.
  • de Korte D; Department of Blood Cell Research, Sanquin Research, Amsterdam, The Netherlands.
  • Nieuwland R; Department of Blood Cell Research, Sanquin Research, Amsterdam, The Netherlands.
  • Kulik W; Department Product and Process Development, Sanquin Blood Bank, Amsterdam, The Netherlands.
  • Vlaar AP; Laboratory for Experimental Clinical Chemistry, Academic Medical Center, Amsterdam, The Netherlands.
Vox Sang ; 112(1): 25-32, 2017 Jan.
Article en En | MEDLINE | ID: mdl-28097705
BACKGROUND AND OBJECTIVES: The accumulation of non-polar lipids arachidonic acid, 5-hydroxyeicosatetraenoic acid (HETE), 12-HETE and 15-HETE during storage of transfusion products may play a role in the onset of transfusion-related acute lung injury (TRALI), a syndrome of respiratory distress after transfusion. MATERIALS AND METHODS: We investigated non-polar lipid accumulation in red blood cells (RBCs) stored for 42 days, plasma stored for 7 days at either 4 or 20°C and platelet (PLT) transfusion products stored for 7 days. Furthermore, we investigated whether transfusion of RBCs with increased levels of non-polar lipids induces TRALI in a 'two-hit' human volunteer model. All products were produced following Dutch Blood Bank protocols and are according to European standards. Non-polar lipids were measured with high-performance liquid chromotography followed by mass spectrometry. RESULTS: All non-polar lipids increased in RBCs after 21 days of storage compared to baseline. The non-polar lipid concentration in plasma increased significantly, and the increase was even more pronounced in products stored at 20°C. In platelets, baseline levels of 5-HETE and 15-HETE were higher than in RBCs or plasma. However, the non-polar lipids did not change significantly during storage of PLT products. Infusion of RBCs with increased levels of non-polar lipids did not induce TRALI in LPS-primed human volunteers. CONCLUSION: We conclude that non-polar lipids accumulate in RBC and plasma transfusion products and that accumulation is temperature dependent. Accumulation of non-polar lipids does not appear to explain the onset of TRALI (Dutch Trial Register - NTR4455).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lesión Pulmonar Aguda / Reacción a la Transfusión / Lípidos Tipo de estudio: Prognostic_studies Límite: Adolescent / Adult / Humans / Male Idioma: En Revista: Vox Sang Año: 2017 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lesión Pulmonar Aguda / Reacción a la Transfusión / Lípidos Tipo de estudio: Prognostic_studies Límite: Adolescent / Adult / Humans / Male Idioma: En Revista: Vox Sang Año: 2017 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido