Changes in the Neurochemical Composition of Motor Neurons of the Spinal Cord in Mice under Conditions of Space Flight.

Porseva, V V; Shilkin, V V; Strelkov, A A; Krasnov, I B; Masliukov, P M

Porseva, V V; Shilkin, V V; Strelkov, A A; Krasnov, I B; Masliukov, P M.

Afiliación

Porseva VV; Department of Normal Physiology and Biophysics, Yaroslavl State Medical University, Ministry of Health of the Russian Federation, Yaroslavl, Russia.
Shilkin VV; Department of Normal Physiology and Biophysics, Yaroslavl State Medical University, Ministry of Health of the Russian Federation, Yaroslavl, Russia.
Strelkov AA; Department of Normal Physiology and Biophysics, Yaroslavl State Medical University, Ministry of Health of the Russian Federation, Yaroslavl, Russia.
Krasnov IB; Laboratory of Gravitational Biology, State Scientific Centre Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia.
Masliukov PM; Department of Normal Physiology and Biophysics, Yaroslavl State Medical University, Ministry of Health of the Russian Federation, Yaroslavl, Russia. mpm@yma.ac.ru.

Bull Exp Biol Med ; 162(3): 336-339, 2017 Jan.

Article en En | MEDLINE | ID: mdl-28091925

RESUMEN

Expression of choline acetyltransferase, 200-kDa neurofilament protein, 28-kDa calbindin, neuronal NO synthase, caspase 3, and Ki-67 in the motor neurons of spinal cord segments T3-T5 in male C57Bl/6 mice after 30-day space flight in the Bion-M1 biosatellite was studied by immunohistochemical methods. Under conditions space flight, the size of motoneurons increased, the number of neurons containing choline acetyltransferase and neurofilaments, decreased, and the number of calbindin-positive neurons increased; motoneurons, expressing neuronal NO synthase and caspase 3 appeared, while Ki-67 was not detected. Fragmentation of neurons with the formation structures similar to apoptotic (residual) bodies was observed in individual caspase 3-positive motoneurons.

Asunto(s)

Neuronas Motoras/metabolismo; Vuelo Espacial; Médula Espinal/metabolismo; Ingravidez; Animales; Apoptosis/genética; Calbindinas/genética; Calbindinas/metabolismo; Caspasa 3/genética; Caspasa 3/metabolismo; Recuento de Células; Tamaño de la Célula; Colina O-Acetiltransferasa/genética; Colina O-Acetiltransferasa/metabolismo; Regulación de la Expresión Génica; Inmunohistoquímica; Antígeno Ki-67/genética; Antígeno Ki-67/metabolismo; Masculino; Ratones; Neuronas Motoras/química; Neuronas Motoras/patología; Proteínas de Neurofilamentos/genética; Proteínas de Neurofilamentos/metabolismo; Óxido Nítrico Sintasa de Tipo I/genética; Óxido Nítrico Sintasa de Tipo I/metabolismo; Médula Espinal/química; Médula Espinal/patología

Palabras clave

immunohistochemistry; microgravity; motoneurons; spinal cord

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vuelo Espacial / Médula Espinal / Ingravidez / Neuronas Motoras Límite: Animals Idioma: En Revista: Bull Exp Biol Med Año: 2017 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Estados Unidos

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