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Perspectives: using polymer modeling to understand the formation and function of nuclear compartments.
Haddad, N; Jost, D; Vaillant, C.
Afiliación
  • Haddad N; CNRS, Laboratoire de Physique, University of Lyon, ENS de Lyon, University of Claude Bernard, 69007, Lyon, France.
  • Jost D; University Grenoble-Alpes, CNRS, TIMC-IMAG lab, UMR 5525, Grenoble, France. daniel.jost@univ-grenoble-alpes.fr.
  • Vaillant C; CNRS, Laboratoire de Physique, University of Lyon, ENS de Lyon, University of Claude Bernard, 69007, Lyon, France. cedric.vaillant@ens-lyon.fr.
Chromosome Res ; 25(1): 35-50, 2017 03.
Article en En | MEDLINE | ID: mdl-28091870
Compartmentalization is a ubiquitous feature of cellular function. In the nucleus, early observations revealed a non-random spatial organization of the genome with a large-scale segregation between transcriptionally active-euchromatin-and silenced-heterochromatin-parts of the genome. Recent advances in genome-wide mapping and imaging techniques have strikingly improved the resolution at which nuclear genome folding can be analyzed and have revealed a multiscale spatial compartmentalization with increasing evidences that such compartment may indeed result from and participate to genome function. Understanding the underlying mechanisms of genome folding and in particular the link to gene regulation requires a cross-disciplinary approach that combines the new high-resolution techniques with computational modeling of chromatin and chromosomes. In this perspective article, we first present how the copolymer theoretical framework can account for the genome compartmentalization. We then suggest, in a second part, that compartments may act as a "nanoreactor," increasing the robustness of either activation or repression by enhancing the local concentration of regulators. We conclude with the need to develop a new framework, namely the "living chromatin" model that will allow to explicitly investigate the coupling between spatial compartmentalization and gene regulation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Modelos Moleculares / Núcleo Celular / Redes Reguladoras de Genes Límite: Animals / Humans Idioma: En Revista: Chromosome Res Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Modelos Moleculares / Núcleo Celular / Redes Reguladoras de Genes Límite: Animals / Humans Idioma: En Revista: Chromosome Res Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Países Bajos