Your browser doesn't support javascript.
loading
Induction of Apoptosis in Normal Human Dermal Fibroblasts Infected with Borrelia burgdorferi Sensu Lato.
Rozwadowska, Beata; Albertynska, Marta; Okla, Hubert; Jasik, Krzysztof P; Swinarew, Andrzej S; Mazurek, Urszula; Dudek, Slawomir; Urbanska-Jasik, Danuta; Poprawa, Izabela.
Afiliación
  • Rozwadowska B; 1 Department of Skin Structural Studies, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice , Poland .
  • Albertynska M; 1 Department of Skin Structural Studies, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice , Poland .
  • Okla H; 1 Department of Skin Structural Studies, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice , Poland .
  • Jasik KP; 2 Institute of Materials Science, Faculty of Computer Science and Materials Science, University of Silesia in Katowice , Poland .
  • Swinarew AS; 1 Department of Skin Structural Studies, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice , Poland .
  • Mazurek U; 2 Institute of Materials Science, Faculty of Computer Science and Materials Science, University of Silesia in Katowice , Poland .
  • Dudek S; 3 Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice , Poland .
  • Urbanska-Jasik D; 4 Department of Pharmacognosy and Phytochemistry, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice , Poland .
  • Poprawa I; 5 Department of Animal Histology and Embryology, Faculty of Biology and Environmental Protection, University of Silesia in Katowice , Poland .
Vector Borne Zoonotic Dis ; 17(4): 237-242, 2017 04.
Article en En | MEDLINE | ID: mdl-28068192
The spirochete Borrelia burgdorferi s.l. can enter into different eukaryotic cells. Intracellular localization of bacteria may cause many changes in different cell pathways like apoptosis-mediated caspase cascade. The present studies focused on gene expression associated with caspase cascade after normal human dermal fibroblasts (NHDF) infection with Borrelia garinii, Borrelia afzelii, and B. burgdorferi s.s. The use of oligonucleotide microarray technique enabled an expression level comparison of genes associated with caspase cascade in NHDF infected with spirochetes. The increased expression of genes associated with caspase cascade was observed in case of CASP5, CASP2, CARD10, CASP10, MALT1, and NLRP1. The decreased expression was observed in case of CASP4, CASP6, and CASP1. The mRNA expression for CASP3 was inhibited in cells infected with three genospecies of Borrelia. However, the intensity of fluorescence was not statistically significant. In addition, cell cultures were fixed and procedure of caspase-3 detection and the TUNEL assay were performed. The in situ caspase-3 detection procedure confirmed the results obtained from microarray analyses. Only several fluorescent signals were observed. Many apoptotic cells were detected in NHDF-infected cultures with all spirochete genospecies found using the TUNEL reaction.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Grupo Borrelia Burgdorferi / Apoptosis / Fibroblastos Límite: Humans Idioma: En Revista: Vector Borne Zoonotic Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS / EPIDEMIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Grupo Borrelia Burgdorferi / Apoptosis / Fibroblastos Límite: Humans Idioma: En Revista: Vector Borne Zoonotic Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS / EPIDEMIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Estados Unidos